Abstract:
Objective To explore the effects of pre-exposure with microwave on γ-rays induced apoptosis, mitochondrion membrane potential (MMP), intracellular Ca2+ concentration and Ca2+-Mg2+-ATPase activity in mouse bone marrow stromal cells.
Methods Primarily cultured mouse bone marrow stromal cells were randomly divided into control group, microwave alone group, γ-rays group and combined exposure group. The microwave alone and combined exposure groups were exposed to 12 μW/cm2 microwave 1 h a day for 7 days. The γ-rays alone and combined exposure groups were subsequently exposed to 5 Gy 60Co γ-rays on the 8th day. Ca2+ concentration and MMP were detected by a flow cytometry (FCM). Apoptosis was detected by Annexin V-FITC/PI, and Ca2+-Mg2+-ATPase activities were analyzed by a commercial kit.
Results There were no significantly differences between different groups in apoptosis and MMP. 24 h after γ-rays irradiation, the Ca2+ concentrations of the microwave alone, the γ-rays alone and the combined exposure groups were statistically higher than those of the control group (P < 0.05), while Ca2+-Mg2+-ATPase activities were statistically lower than those of the control group (P<0.05). Compared with the γ-rays alone group, Ca2+ concentrations of the combined exposure group significantly decreased (P < 0.05)and Ca2+-Mg2+-ATPase activity statistically increased (P < 0.05).
Conclusion Under the experimental condition, microwave radiation (900 MHz, 12 μW/cm2)and 5 Gy γ-rays did not induce evident changes in apoptosis and MMP; but did induce an increase of Ca2+ concentrations and a decrease of Ca2+-Mg2+-ATPase activity. Pre-exposure with microwave could significantly decrease γ-rays induced changes in Ca2+ concentration and Ca2+-Mg2+-ATPase activity.
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