白介素-1A(<i>IL-1A</i>)和<i>IL-1B</i>基因多态性与冠心病关系的病例-对照研究

何美安; 周莉; 程龙献; 张晓敏; 郭欢; 王芳; 邬堂春

白介素-1A(IL-1A)和IL-1B基因多态性与冠心病关系的病例-对照研究

Case-control Study on the Associations of Polymorphisms in Interleukin-1A(IL-1A) and IL-1B Genes with Coronary Heart Disease

摘要

摘要:
目的研究白介素-1A (interleukin-1A,IL-1A)和IL-1B基因多态性与冠心病(CHD)的关系。

方法采用病例-对照研究,按照WHO的CHD诊断标准,选取CHD病例1 003人,年龄和性别频率匹配的对照1 003人。使用标准化的调查表获得研究对象的基本资料;采用ABI 7900HT的Taqman基因分型技术检测基因多态性。

结果 IL-1A基因外显子上的多态性位点rs17561(ala114ser)与CHD有关。与GG相比,携带TT基因型者患CHD的危险性为GG的3倍。未发现IL-1B基因上的4个多态性位点(SNPs)与CHD有关。进一步的单倍型研究显示单倍型在CHD组和对照组差异没有统计学意义。

结论基因IL-1A上的多态性位点rs17561与CHD有关,IL-1B上的4个SNPs与CHD无关。

Abstract:
Objective To explore the associations of polymorphisms in interleukin-1A (IL-1A) and IL-1B genes with coronary heart disease (CHD).

Methods CHD cases were diagnosed according to WHO criteria at three of the major hospitals in Wuhan. A total of 1 003 patients with confirmed CHD and 1 003 age-and sex-matched controls were included in the present study. Participants' information and data were obtained by a standard questionnaire. Polymorphisms of IL-1A and IL-1B genes were detected by Taqman single nucleotide polymorphism (SNP)allelic discrimination by means of an ABI 7900HT.

Results It indicated that SNP rs17561(ala114ser) in exon of IL-1A gene was associated with higher risk of CHD. Compared with GG genotype, subjects with TT had more than 3-fold risk of CHD. Four SNPs in IL-1B gene were not significantly associated with CHD. Further haplotype analysis did not find any significant association with CHD.

Conclusion These results suggested that SNP rs17561 in IL-1A gene was associated with higher risk of CHD. This finding needed to be validated in further studies.

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