Abstract:
Objective To investigate gene polymorphisms of transforming growth factor-beta1(TGF-β1)-509 C/T in patients with coal worker's pneumoconiosis (CWP).
Methods Total of 110 patients with documented CWP and 110 workers exposed to dust and 110 local residents without dust exposure as control group were recruited. The TGF-β1-509 C/T genotypes were determined by PCR-restriction fragment length polymorphism of vein blood samples.
Results The average ages of CWP group, the dust exposure group and the control group were (48.30& #177;13.16) (Mean& #177;SD),(43.86& #177;4.20), and (41.73& #177;5.45)years respectively; The average working ages of the CWP group and the coal dust exposure group were (17.40& #177;7.84)years (Mean& #177;SD)and (16.95& #177;6.10) years respectively. 48.2% of the CWP group and 49.1% of the dust exposure group were drifters. The durations of smoking varied in the CWP group, the dust exposure group and the control group(10.48& #177;10.12)years (Mean& #177;SD),(11.34& #177;10.48)years,(12.65& #177; 11.53)years, respectively.The length of dust expsure and job title distribution were found no statistically difference between worker's groups. Smoking ages were not difference in all three groups. The percentage of TGF-β1-509 C/T genotype polymorphism were 51.8%、30.9% and 25.5% for the CWP group, the dust exposure group and the control group respectively.The allele frequency of T in the CWP group, the dust exposure group and the control group were 40.9%、23.2%、19.1% respectively. Both TGF-β1-509 C/T genotypes and alleles frequencies were significantly higher in the CWP patients than in the controls (P < 0.05).
Conclusion TGF-β1-509 C/T polymorphisms distributions were different in workers with CWP and residents without dust exposure.
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