p,p'-DDD对人乳腺癌细胞的增殖作用

Study of the Proliferation Effect of DDD on Mammary Cancer Cell MCF-7

摘要:
目的观察对,对-二氯二苯基二氯乙烷(p,p'-DDD)对人乳腺癌(MCF-7)细胞增殖的影响。

方法采用体外培养 MCF-7细胞增殖试验检测 DDD的类雌激素活性, 并用生长曲线对其作用机制进行初步探讨。

结果 DDD染毒剂量在 3& #215;10^-7 mol/L、3& #215;10^-6 mol/L时, 存在刺激 MCF-7细胞增殖的类雌激素活性(P < 0.05), 在 3& #215;10^-6 mol/L剂量时其增殖效应最大。细胞生长曲线方面, DDD组在第 3、5、7天各时间段细胞数均明显高于溶剂对照组(P < 0.05)。

结论 DDD在 3& #215;10^-7 mol/L、3& #215;10^-6 mol/L具有刺激 MCF-7细胞增殖的类雌激素活性。

Abstract:
Objective To study the proliferation effect of p,p'-DDD on human mammary cancer cell MCF-7.

Methods We detected estrogenic activity of DDD using the MCF-7 cell proliferation experiment in vitro, and analysed its mechanism by means of growth curve.

Results DDD in the dose of 3& #215;10^-7 mol/L and 3& #215;10^-6 mol/L could stimulate the MCF-7 cell proliferation and produce estrogenic activity, and the most proliferation effect dose was 3& #215;10^-6 mol/L. Cell cycle analysis of cell proliferation index indicated that DDD group was significantly higher than the control group at the third, fifth, and seventh day.

Conclusion DDD in the dose of 3& #215;10^-7 mol/L and 3& #215;10^-6 mol/L could stimulate the MCF-7 cell multiplication.