Abstract:
Objective To study the percutaneous penetration of perfluorooctanoate acid(PFOA)and perfluorooctane sulfonate(PFOS)as well as its absorption characteristics through excised rat skin in vitro.
Methods Using finite dose of 10μmol/L PFOA and PFOS mixed solution as body fluids combined with Franz diffusion cells and HPLC-MS method, the differences of accumulative permeating amounts between PFOA and PFOS were measured, and their steady-state penetration rates, lag time and permeability coefficients were calculated.
Results PFOA and PFOS could be detected in receptor fluids at each time point, the calculated PFOA and PFOS cumulative permeation amounts at 12 h, steady-state penetration rates, lag time, permeability coefficients were(4.97& #177;1.80)and(1.76& #177;1.06)μg/cm2(0.48& #177;0.19)and(0.16& #177;0.06)μg/cm2& #183;h,(0.31& #177;0.18)and(0.50& #177;0.06)h(, 11.48& #177;4.51)& #215;10-2 and(3.17& #177;1.22)& #215;10-2 cm/h respectively.
Conclusion PFOA and PFOS were able to conduct percutaneous absorption, the formers penetrated at a faster and higher rate with significant shorter lag time. The study identified the characteristics of PFOA and PFOS percutaneous absorption, provided basic research data for exposure and risk assessment.
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