Abstract:
Objective To explore the regulation of cell cycle and target gene in lung cancer A549 cells by microRNA (miRNA/miR) 1908.
Methods Up-regulated miR-1908 expression in A549 cells was induced by lentivirus transfection. Then, MTT and flow cytometry were applied to detect the influence of miR-1908 on cell proliferation, apoptosis, and cell cycle of A549 cells. DIANATOOLs and KEGG databases were used to analyze the bioinformatics of miR-1908 and explore potential signaling pathways and target genes. RT-qPCR and Western blot were applied to detect the miR-1908 regulation on both mRNA and protein expression le vels of its predicted target gene.
Results Compared with the negative control group, the up-regulation of miR-1908 in the transfection group caused arrested G2 phase and shortened S phase of A549 cells (P < 0.05), but did not induce significant changes in cell proliferation and apoptosis (P > 0.05). miR-1908 was mainly related to MAPK signaling pathway, and protein phosphatase 5 (PP5) was an important potential target gene. Compared with the negative control group, the up-regulation of miR-1908 in the transfection group increased PP5 protein level (P < 0.05), but not its mRNA expression level (P>0.05).
Conclusion miR-1908 may affect the cell cycle of A549 cells by regulating PP5 positively or indirectly. The results help further understanding the role and mechanism of miRNAs in lung cancer cells and provide a novel clue for studying miRNAs in lung cancer.
下载:
