Abstract:
Objective To develop an ultrasensitive performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for simultaneous determination of eleven hydroxylated metabolites of polycyclic aromatic hydrocarbons (OH-PAHs) in human urine, including 2-hydroxynaphthalene, 1-hydroxynaphthalene, 3-hydoxyfluorene, 2-hydoxyfluorene, 2-hydroxyphenanthrene, 1-hydroxyphenanthrene, 1-hydroxypyrene, 3-hydroxychrysene, 6-hydroxychrysene, 9-hydoxybenza pyrene, and 3-hydoxybenza pyrene.
Methods The urine samples were hydrolyzed overnight for 12h first, then purified and enriched by Envi-18 solid phase extraction cartridges (3mL, 500mg), concentrated by nitrogen blowing, separated by Symmetry C18 column (250mm×4.6mm, 5 μm), and finally determined with UPLC-MS/MS (8050).
Results The eleven OH-PAHs showed a good linearity in a concentration range of 0.002-200 μg/L and the correlation coefficients were all higher than 0.99. The recovery rates were 71.4%-109.4%, the relative standard deviations were 2.7%-11.6%, and the limits of detection were between 0.001 and 0.040μg/L.
Conclusion The established method is applicable to analyze eleven OH-PAHs in urine simultaneously. The method is sensitive and reproducible with high precision and good recoveries, and it is suitable for the biomonitoring of PAHs exposure.
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