Abstract:
Objective To study the effects of different oils on intestinal microbiota.
Methods C57BL/6 male mice (6 weeks old, 16-18 g) were divided into 7 groups with 12 mice in each group. The control group was fed with an ordinary diet with a 10% fat-energy ratio, and the other groups were fed with a high-fat diet (D12451) added with lard, soybean oil, 24 degrees palm oil, 33 degrees palm oil, mixture oil (soybean oil and palm oil with a mass ratio of 0.9:1), and olive oil, respectively. After a 12-week diet intervention, blood samples were collected from the orbit of mice to detect total cholesterol and triglyceride. Residual waste was removed from colon to conduct 16S rRNA amplicon sequencing for cluster analysis and speciation analysis. MetaStat was used to analyze species abundance, obtain P value and q value (adjusted P value), and screen species with significant differences.
Results The total cholesterol and triglyceride of the experimental groups were higher than those of the control group. The mixed oil group showed a higher level of relative abundance of Actinobacteria than the control group (q < 0.05). The lard group and the 33 degrees palm oil group showed higher levels of relative abundance of Verrucomicrobia than the control group (q < 0.05).
Conclusion A long-term high-fat diet could affect the intestinal flora of mice. Soybean oil, olive oil, and 24 degrees palm oil do not cause abnormalities in intestinal microbiota.
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