Abstract:
Objective To establish a method based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to assess six kinds of environmental endocrine disruptors including four phthalate metabolites (MPAEs), bisphenol A (BPA), and nonylphenol (NP) in the urine of women of reproductive age in Nanjing.
Methods Pretreatment and UPLCMS/MS analytical method was established for MPAEs, BPA, and NP. The accuracy, intra-day precision, and inter-day precision of the method were assessed. Then 120 urine samples collected from women of reproductive age in Nanjing were measured for concentrations of phthalate metabolites, BPA, and 4-n-NP.
Results All the analytes were linear over the range of 1-100 μg/L (r2>0.998). The limits of detection were 0.1 μg/L for mono-n-butyl phthalate (MBP),mono-isobutyl phthalate (MiBP), mono-benzyl phthalate (MBzP), and mono-2-ethylhexyl phthalate (MEHP), and 0.3 μg/L for BPA and 4-n-NP. The recoveries by solid phase extraction of all analytes ranged from 92.3% to 103.5%; the intra-day and inter-day precisions were all less than 10%. The median concentrations of MBP, MiBP, MBzP, MEHP, BPA, and 4-n-NP were 5.04, 2.10, 0.42, 5.24, 1.32, and 1.47 μg/L, respectively.
Conclusion The sensitive and accurate method is suitable for the determination of MPAEs, BPA and 4-n-NP in urine. Women of reproductive age present high positive rates of MBP, MiBP, MBzP, MEHP, and BPA.
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