应用无线遥测技术研究褪黑素的降压作用

孙金鹏; 邵光方; 王旭; 秦粉菊; 童建

doi:10.13213/j.cnki.jeom.2015.14509

应用无线遥测技术研究褪黑素的降压作用

Application of Wireless Telemetry Technology to Antihypertension Effect of Melatonin

摘要

摘要:
目的探讨不同昼夜时点给予褪黑素对自发性高血压大鼠血压及血清激素水平的影响,对褪黑素的降压机制进行初步研究。

方法 20周龄自发性高血压大鼠6只,分别在收缩压峰值和谷值前1 h给予褪黑素(10 mg/kg),采用无线遥测方法,动态监测大鼠收缩压、舒张压、平均压。腹腔注射褪黑素0、1、2、3 h后取血,采用自身对照的方法测定并比较褪黑素注射前后对应时点大鼠血压和血清内皮素、一氧化氮(NO)水平。

结果与自身对照比较,注射褪黑素2 h后,峰、谷值组大鼠收缩压下降率分别为11.56%、10.45%,舒张压下降率分别为12.66%、16.63%,平均压下降率分别为12.02%、12.88%;注射褪黑素后1 h,NO上升率分别为55.44%、79.1%,内皮素下降率分别为14.22%、29.04%。

结论褪黑素对自发性高血压大鼠有降压作用,部分原因可能是通过降低内皮素水平,提高NO水平而实现。

Abstract:
Objective To apply wirelss telemetry technology to evaluation on the effect of circadian administration of melatonin on blood pressure and serum endothelin (ET) in spontaneously hypertensive rats (SHR),amd explore the antihypertensive mechanism of melatonin.

Methods Melatonin solution (10 mg/kg) were intraperatoneally injected into six 20-week-old SHRrats one hour before the peak and the trough of systolic blood pressure (SBP) rhythm respectively,and systolic,diastolic (DBP),and mean blood pressures (MBP) were continuously monitored by wirelss telemetry technology for comparison.Blood samples were takento measure nitric oxide (NO) and ET at 0,1,2,and 3 h after the melatonin injection for before-and-after comparison.

Results After melatonin treatment for 2 h,the peak and the trough injection groups were decreased by 11.56% and 10.45% for SBP,12.66% and 16.63% for DBP,12.02% and 12.88% for MBP,respectively.After melatonin treatment for 1 h,the levels of NO of the two groups increased by 55.44% and 79.1%,but the levels of ET decreasded by 14.22% and 29.04%.

Conclusion Melatonin treatment could result in a lower blood pressure in the SHR rats,partially due to mediation of up-regulated NO and down-regulated ET levelsgroup

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