葡萄籽原花青素对NaF致雄性小鼠肝脏氧化损伤的拮抗作用

牛强; 谢馨瑶; 刘雪娇; 何芳; 丹增卓玛; 李述刚

doi:10.13213/j.cnki.jeom.2015.14462

葡萄籽原花青素对NaF致雄性小鼠肝脏氧化损伤的拮抗作用

Antagonistic Effect of Grape Seed Proanthocyanidin Extract on Male Mice Liver Oxidative DamageInduced by Excessive Sodium Fluoride

摘要

摘要:
目的观察葡萄籽原花青素(GSPE)对氟化钠(NaF)致雄性小鼠肝脏组织氧化损伤的拮抗作用。

方法将40只健康性成熟昆明种雄性小鼠随机分为4组:对照组(0 mg/kg)、NaF组(20 mg/kg)、GSPE组(200 mg/kg)及NaF+GSPE组NaF(20 mg/kg)+GSPE(200 mg/kg),每组10只动物。灌胃染毒,每天1次,连续染毒5周。颈椎脱臼处死,采集肝脏组织样本,计算肝脏脏器系数,检测肝脏组织中谷丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和超氧化物歧化酶(SOD)活性,谷胱甘肽(GSH)和丙二醛(MDA)含量以及总抗氧化能力(T-AOC)水平。

结果各组肝脏脏器系数组间比较,差异有统计学意义(P < 0.05);NaF组高于对照组(P < 0.05),(NaF+GSPE)组低于NaF组(P < 0.05)。各组AST、ALT含量组间比较,差异有统计学意义(均P < 0.05);NaF组和(NaF+GSPE)组高于对照组(P < 0.05),GSPE组和(NaF+GSPE)组低于NaF组(P < 0.05)。各组GSH、SOD、MDA和T-AOC含量组间比较,差异有统计学意义(均P < 0.05);与对照组相比,NaF组和(NaF+GSPE)组小鼠肝脏中GSH、SOD和T-AOC含量较低(P < 0.05),MDA含量较高(P < 0.05);与NaF组相比,GSPE组和(NaF+GSPE)组GSH、SOD和T-AOC含量较高(P < 0.05),MDA含量较低(P < 0.05)。析因分析显示:NaF与GSPE对AST、ALT、SOD活力、MDA含量,T-AOC水平存在拮抗作用。

结论 GSPE对NaF致雄性小鼠肝脏氧化损伤具有拮抗作用。

Abstract:
Objective To observe the antagonistic effect of grape seed proanthocyanidin extract (GSPE) on oxidative damage of male mice which were treated with excessive sodium fluoride (NaF).

Methods A total of 40 healthy and sexually mature male mice were randomly divided into four groups:control,NaF (20 mg/kg),GSPE (200 mg/kg),and NaF (20 mg/kg)+GSPE (200 mg/kg) groups.The animals were fed with deionized water supplemented with non-NaF,NaF,GSPE,or NaF+GSPE for consecutive five weeks,and sacrificed by cervical dislocation to collect liver tissue samples.We calculated the liver/body weight index and measured the activities of alanine aminotransferase (ALT),aspartate aminotransferase (AST),and superoxide dismutase (SOD) and the levels of glutathione (GSH),malondialdehyde (MDA),and total anti-oxidant capacity (T-AOC).

Results The differences in liver/body weight index were statistically significant among the experiment groups (P < 0.05).Compared with the control group,the liver/body weight index of the NaF group was increased;compared with the NaF group,that of the NaF+GSPE group was decreased.The differences in AST and ALT activities among the experiment groups were statistically significant (all Ps < 0.05).Compared to the control group,the activities of AST and ALT of the NaF group and the NaF+GSPE group were increased (P < 0.05);compared to the NaF group,the GSPE group and the NaF+GSPE group were decreased (P < 0.05).The differences in GSH,SOD,MDA,and T-AOC contents among the experiment groups were also statistically significant (all Ps < 0.05).Compared with the control group,the contents of GSH,SOD,and T-AOC of the NaF group and the NaF+GSPE group were decreased (P < 0.05),but the MDA content was increased (P < 0.05);compared with the NaF group,the contents of GSH,SOD,and T-AOC of the GSPE group and theNaF+GSPE group were increased (P < 0.05),but the MDA content was decreased (P < 0.05).The results of factorial analysis showed an antagonistic interaction between NaF and GSPE on AST,ALT,SOD activities,MDA content,and T-AOC level.

Conclusion GSPE could antagonize the oxidative damage induced by excessive NaF in male micegroup

HTML全文

参考文献(0)

施引文献

补充资料

审稿意见

勘误/撤稿