纳米二氧化钛致小鼠肝组织DNA氧化损伤的研究

宋明芬; 王玉文; 王翀; 章隆; 彭开良; 吕雅英

doi:10.13213/j.cnki.jeom.2015.14411

纳米二氧化钛致小鼠肝组织DNA氧化损伤的研究

Oxidative DNA Damage in Liver of Mice Induced by Titanium Dioxide Nanoparticles

摘要

摘要:
目的探讨纳米二氧化钛对小鼠DNA的氧化损伤作用。

方法将20只雌性小鼠随机分成对照组和低、中、高3个染毒组,每组5只动物,采用尾静脉注射染毒;各组纳米二氧化钛染毒剂量分别为0、100、200、400 mg/kg体重;动物染毒后24 h处死,碘化钠法提取小鼠肝、肺、肾、骨髓、大脑组织中的DNA,采用高效液相色谱-ECD(HPLC-ECD)法测定各组织中的8-羟基脱氧鸟苷(8-OHdG),观察纳米二氧化钛对不同组织DNA的氧化损伤情况。

结果低、中、高3个染毒组的肝脏DNA水平分别是每106个脱氧鸟苷(dG)中含8-OHdG个数(1.07& #177;0.11)、(1.49& #177;0.13)、(1.39& #177;0.18),高于对照组(0.82& #177;0.06),差异有统计学意义(P < 0.01);各染毒组其他脏器组织中8-OHdG水平与对照组比较,差异无统计学意义(P > 0.05)。

结论纳米二氧化钛可导致小鼠肝脏DNA氧化损伤增加,对肺、肾、骨髓、大脑中的DNA氧化损伤未见影响。

Abstract:
Objective To examine the oxidative DNA damage induced by titanium dioxide nanoparticles in mice.

Methods Twenty female mice were randomly divided into four groups and administered with titanium dioxide nanoparticles (0,100,200,400 mg/kg body weight) by intravenous injection.All the mice were sacrificed at the 24 h and samples of liver,lung,kidney,bone marrow,and brain were harvested for 8-hydroxyguanosine (8-OHdG) detection.

Results The counts of 8-OHdGper 106 dG in liver DNA of three titanium dioxide nanoparticle-treated groups (1.07& #177;0.11,1.49& #177;0.13,and 1.39& #177;0.18) wereobviously higher as compared with the counts of the control group (0.82& #177;0.06)(P < 0.01).No change of 8-OHdG level was observedin the other tested tissues (P > 0.05).

Conclusion Titanium dioxide nanoparticles could elevate the oxidative DNA damage inmice liver but not in lung,kidney,bone marrow,and braingroup

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