Abstract:
Objective To explore the role of aluminum in the regulation of a disintegrin and metalloproteinase-10(α-ADAM10) via retinoic acid receptors-α(RAR-α).
Methods Healthy male SD rats (n=40) were randomly divided by weight into four groups:control group(saline),low-dose group(Al3+ 0.4 mg/kg& #183;d),mid-dose group(Al3+ 0.8 mg/kg& #183;d),and high-dose group(Al3+ 1.2 mg/kg& #183;d) by intraperitoneal injection for five consecutive days per week in a period of two months.Rats were neutralized at the end of the two-month exposure,and cerebral cortex and hippocampus samples were dissected from each rat and stored at-80℃.Graphite furnace atomic absorption spectrometry was used to detect aluminum content.Western blot was used to determine the expressions of RAR-α and α-ADAM10.
Results With the exposure dose increasing,the aluminum contents in cerebral cortex and hippocampus were significantly increased.Compared with the 0.0mg/kg group,α-ADAM10 expression was significantly decreased in the 0.4 mg/kg and above groups in a dose-response manner (P < 0.05).A decreasing trend was also observed for RAR-α expression in cerebral cortex in the groups treated with 0.4 mg/kg and more in a dose-response manner (P < 0.05),while only the 1.2 mg/kg group showed a significant decrease in RAR-α expression in hippocampus compared with the other groups (P < 0.05).
Conclusion Aluminum may inhibit α-ADAM10 secrete enzymes expression through blocking RAR-α in rats with subchronic exposure to aluminum.
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