CXC趋化配体10和γ-干扰素在矽肺纤维化大鼠肺组织中的表达
Expression of C-X-C Motif Chemokine 10 and Interferon-γ in Fibrosis Lung Tissue of Silica Exposed Rats
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摘要:
目的 比较CXC 趋化配体10(CXCL10)和γ-干扰素(IFN-γ)在实验性矽肺大鼠肺组织中的表达,为矽肺发病机制的研究提供新的思路。 方法 12 只健康雄性SD大鼠随机分为实验组和对照组,每组6 只,采用非暴露气管灌注法分别对实验组和对照组大鼠染矽尘和灌注生理盐水,实验组大鼠一次性气管注入50 mg/mL SiO2 粉尘1 mL,对照组大鼠注入同样体积的生理盐水。染尘40 d 后病理切片证实矽肺模型构建成功后,利用实时荧光定量聚合酶链反应和免疫组织化学法分别测定两组大鼠肺组织中CXCL10 和IFN-γ 基因及蛋白表达水平,比较二者的差异。 结果 实时荧光定量PCR显示两组CXCL10 和IFN-γ 基因表达水平没有差异。但是免疫组织化学结果发现,矽肺大鼠肺组织中CXCL10 和IFN-γ 蛋白表达水平均高于生理盐水对照组,差异具有统计学意义(P < 0.05)。 结论 CXCL10 和IFN-γ 在纤维化实验组织中的表达高于正常肺组织,提示两个因子可能在矽肺纤维化过程中发挥着重要作用。 Abstract:Objective To compare the expression of C-X-C motif chemokine 10 (CXCL10) and interferon-γ (IFN-γ) in lung tissue of experimental silicosis rats, and to provide a new way for study on pathogenesis of silicosis. Methods Twelve healthy male SD rats were divided into an experimental group and a control group which were administered with silica and saline respectively by non-exposed tracheal perfusion. A silicosis rat model was successfully established after 40 days of exposure and confirmed by pathological observation. Gene and protein expressions of CXCL10 and IFN-γ were detected and compared between the two groups by real-time quantitative polymerase chain reaction and immunohistochemistry, respectively. Results Compared with the control group, the gene expressions of CXCL10 and IFN-γ in the silicosis group showed no significant difference. However, the protein expressions of CXCL10 and IFN-γ of the silicosis group were remarkably higher than those of the control group (P < 0.05). Conclusion CXCL10 and IFN-γ expressions in silicosis tissue are higher than those in normal lung tissue, indicating that these 2 cytokines may play an important role in the development of pulmonary fibrosis.
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