双酚A经口亚急性染毒对雌性小鼠卵巢结构和功能的影响
Effects of Bisphenol A on Ovary Function and Structure of Female Mice
-
摘要:
目的 研究双酚A(bisphenol A,BPA)经口亚急性染毒对雌性小鼠卵巢结构和功能的影响,探讨BPA是否有可能引起卵巢早衰。 方法 5 周龄SPF 清洁级昆明雌性小鼠经检疫1 周后,将小鼠随机分为溶剂对照组和低、中、高剂量组,每组10 只小鼠。低、中、高剂量组的染毒剂量分别为40、120、240 mg/kg,配成相应的玉米油溶液,按0.4％体重灌胃,对照组用等体积玉米油灌胃。染毒25 d 后观察其组织病理学,用酶联免疫吸附法(ELISA)测量血清雌二醇(E2)、小鼠抗卵巢抗体(AOAb)水平,用免疫组化方法观察bax、bcl-2 表达情况。 结果 与对照组相比,低、中、高染毒剂量组的小鼠E2 浓度升高(P<0.05),分别为(13.79& #177;2.36)、(19.68& #177;1.34)、(22.35& #177;1.26)pg/mL;AOAb 浓度亦升高(P<0.05),分别为(3.11& #177;0.24)、(3.99& #177;0.56)、(4.51& #177;0.25)ng/mL;bax 表达增加,bcl-2 表达减少。 结论 BPA可引起小鼠卵巢功能与结构明显变化以及自身免疫反应,但尚不能证明其引起昆明小鼠卵巢早衰。 Abstract:Objective To investigate the effects on ovary function and structure of female mice by oral administration of bisphenol A (BPA) and to explore BPA's relevance to premature ovarian failure. Methods Kunming female mice (SPF grade) at 5 weeks of age were randomly divided into control group (administered with corn oil) and BPA treatment groups (40, 120, and 240 mg/kg), 10 mice for each group. After intragastric administration for 25 d, histological changes of ovary tissues were observed; estradiol and antiovary antibody were measured by enzyme-linked immunosorbent assay (ELISA); expressions of bax and bcl-2 protein were examined by immunohistochemistry. Results The levels of estradiol in the 40, 120, and 240 mg/kg BPA treatment groups were (13.79& #177;2.36), (19.68& #177;1.34), and (22.35& #177;1.26) pg/mL, respectively, and were significantly higher than that in the control group (P<0.05). The levels of antiovary antibody in the 3 exposure groups were (3.11& #177;0.24), (3.99& #177;0.56), and (4.51& #177; 0.25) ng/mL, respectively, significantly higher than that in the control group (P<0.05). The expression of bax protein in each BPA treatment group was significantly elevated (P<0.05), while that of bcl-2 were down-regulated (P<0.05). Conclusion Autoimmunity response and obvious changes in ovary function and structure of female mice are recorded after exposure to BPA, but need further evidence to support the association between BAP and premature ovarian failure.
下载:
