混合化学改进剂用于测定血铅和尿铅的石墨炉原子吸收法
Using Mixed Chemical Modifiers in Determination of Lead in Blood and Urine by Graphite Furnace Atomic Absorption Spectrometry
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摘要:
目的 研究混合化学改进剂用于石墨炉原子吸收法测定血铅和尿铅的方法。 方法 通过比较5 种化学改进剂的作用效果,优化石墨炉升温程序的测试条件,直接稀释血样、尿样进行铅含量的测定。 结果 以0.2%磷酸二氢铵+0.05%硝酸镁为化学改进剂测血铅,以4%磷酸二氢铵+6%抗坏血酸为化学改进剂测尿铅,血铅和尿铅测定线性范围分别为3.0~100.0 μg/L 和6.7~80.0 μg/L,检出限分别为0.9 μg/L 和2.0 μg/L,相对标准偏差均小于5%,加标回收率为92.1%~104.0%。 结论 使用混合化学改进剂测定血铅和尿铅,灵敏、准确、重现性好,费用低廉,易于普及,适合大批量样品的检测。 Abstract:Objective Using mixed chemical modifiers to improve the determination of lead in blood and urine by graphite furnace atomic absorption spectrometry. Methods Through comparing the effects of five chemical modifiers and optimizing the test condition of graphite furnace, lead in blood and urine were determined by directly diluting. Results A mixture of 0.2% ammonium dihydrogen phosphate and 0.05% magnesium nitrate solution was used as chemical modifier for the blood samples containing lead. A mixture of 4% ammonium dihydrogen phosphate and 6% ascorbic acid solution was used as chemical modifier for the urine samples containing lead. The linear ranges of blood and urine lead determination respectively were 3-100 μg/L and 6.7-80 μg/L respectively. The detection limits were 0.9 μg/L and 2.0 μg/L respectively. The relative standard deviations (RSD) were less than 5%. The addition standard recovery rates were 92.1%-104.0%. Conclusion Using mixed chemical modifier to facilitate lead determination in blood or urine is sensitive, accurate and of good reproducibility, low cost, and ease to popularize. This method is suitable for the detection of bulk samples.
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