负压二级生物安全实验室防护措施设计分析与评价
Design and Evaluation of Protection System for Biosafety Laboratory Level-2 with Negative Air Pressure
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摘要:
目的 指导负压二级生物安全实验室防护措施设计。 方法 就负压二级实验室建造中的突出问题,结合工程实例,通过实验工艺、功能分区、平面布局、人流与物流组织、通风空调方式、气流组织、环境参数等关键因素分析,提出实施意见。 结果 应设缓冲间;核心间/动物间形态宜规整;宜设更衣和准备间。宜采用直流系统;整体气流由清洁区流向核心/动物间,核心/动物间气流由相对清洁处流向污染源。气压梯度不小于10 Pa;负压名义值:核心间,包括合并动物间的宜-20 Pa;独立动物间宜-30 Pa。 结论 设计不仅应符合生物安全理念和实验工艺要求,而且应符合暖通、建筑等原理与要求。该指导性意见对科学建设实验室具有积极意义,并已在许多工程中得以验证。 Abstract:Objective To guide the design of protection system for biosafety laboratory level-2(BSL-2) with negative air pressure. Methods The suggestions were proposed through summarized the significant problems in laboratory construction,experiment process,function isolation,floor layout,organization of people and logistics,ventilation,air flowand other environment parameters. Results Buffer room and dressing room should be included in BSL-2 as well as preparation room.The form of animal room should be regulated and should apply co-current system.The entirely current should flow from pristine area to animal room and air current in animal room should flow form relative cleaning to pollution zone.Baric gradient should not be less than 10Pa.The value of negative pressure should be-20 Pa in core room included combination animal room and-30 Pa in the independence animal room. Conclusion The design must consistent with biosafety and requirement of experiment process but also should agree with the need of heating ventilation and construction principle.In the paper,the proposed suggestions can guide the construction of laboratory and have been confirmed in the many projects.
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