张淑坤, 张鑫生, 王建国, 齐文斌. 高海拔缺氧对链脲佐菌素致大鼠胰岛损害程度的影响[J]. 环境与职业医学, 2011, 28(9): 543-546.
引用本文: 张淑坤, 张鑫生, 王建国, 齐文斌. 高海拔缺氧对链脲佐菌素致大鼠胰岛损害程度的影响[J]. 环境与职业医学, 2011, 28(9): 543-546.
ZHANG Shu-kun , ZHANG Xin-sheng , WANG Jian-guo , QI Wen-bin . Influence of Hypoxia on Islet Morphology of Streptozotocin-induced Diabetic Rats at High Altitude[J]. Journal of Environmental and Occupational Medicine, 2011, 28(9): 543-546.
Citation: ZHANG Shu-kun , ZHANG Xin-sheng , WANG Jian-guo , QI Wen-bin . Influence of Hypoxia on Islet Morphology of Streptozotocin-induced Diabetic Rats at High Altitude[J]. Journal of Environmental and Occupational Medicine, 2011, 28(9): 543-546.

高海拔缺氧对链脲佐菌素致大鼠胰岛损害程度的影响

Influence of Hypoxia on Islet Morphology of Streptozotocin-induced Diabetic Rats at High Altitude

  • 摘要: 目的 通过观察西宁(低海拔)、木里(高海拔)地区链脲佐菌素(streptozotocin, STZ)糖尿病大鼠胰岛形态学特点,探讨高原缺氧环境对STZ糖尿病大鼠胰岛形态学影响。

    方法 大鼠随机分成西宁、木里正常组;西宁、木里造模组。造模组大鼠空腹12h后腹腔注射STZ,以随机血糖 ≥ 16.7mmol/L为造模成功;采用免疫组织化学染色和原位末端转移酶标记技术(TdT-mediated dUTP nick end labeling, TUNEL)细胞凋亡检测分析胰岛面积、胰岛β细胞面积、平均光密度、平均累积光密度(integrated optical density, IOD)和胰岛细胞凋亡率。

    结果 木里糖尿病组血糖水平低于西宁糖尿病组;糖尿病大鼠胰岛数量减少、萎缩、形状不规则,胰岛细胞变性,胰岛内β细胞分布稀少,胰岛染色颗粒较少,着色浅,凋亡细胞较正常组多;木里糖尿病组在β细胞面积、β细胞面积/胰岛面积和IOD上显著高于西宁糖尿病组。

    结论 高海拔STZ糖尿病大鼠血糖较低;胰岛β细胞受损较轻;高原缺氧环境可能对STZ糖尿病大鼠胰岛β细胞有一定的保护作用。

     

    Abstract: Objective To discusses hypoxia influence on islet morphology of streptozotocin (STZ)-induced diabetic rats through observing islet morphology characteristics and measuring morphological index of Xi'ning and Muli diabetic rats.

    Methods Rats were randomly divided into Xi'ning normal group,Muli normal group,Xi'ning diabetic group and Muli diabetic group randomly.Rats of diabetic groups were intraperitoneally injected STZ after a 12-hour-fast.Animal models considered successful if blood sugar levels of rats were higher than 16.7 mmol/L.Islet area,beta cell area,light density,IOD and number of apoptotic islet cell were measured by immunohistochemical staining and TUNEL apoptosis test.

    Results The blood glucose levels of Xi'ning STZ rats were higher than that of the Muli STZ rats.The islet number of STZ rats reduced,islet atrophy with islet cell degeneration was also observed.The beta cells of the STZ rats reduced,insulin dyeing showed shallow.The apoptotic cells in the islets of STZ rats were more than the normal rats,and distributed uniformly.The Xi'ning diabetic rats and the Muli diabetic rats diabetes significantly different between the β cell area,the β cells area/islet size and the average IOD.

    Conclusion Islet hypoxia tolerance,faster glucose uptake and increased insulin sensitivity are likely explanations for STZ diabetic rats at high altitude showing lower blood sugar,minor damages to beta cells.

     

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