党占翠, 宋琦如, 李吴萍, 杨文海, 吴向林. IL-12B+1188A/C基因多态性与宁夏汉族煤工尘肺易感性研究[J]. 环境与职业医学, 2011, 28(9): 535-538.
引用本文: 党占翠, 宋琦如, 李吴萍, 杨文海, 吴向林. IL-12B+1188A/C基因多态性与宁夏汉族煤工尘肺易感性研究[J]. 环境与职业医学, 2011, 28(9): 535-538.
DANG Zhan-cui , SONG Qi-ru , LI Wu-ping , YANG Wen-hai , WU Xiang-lin . Gene Polymorphisms of Interleukin-12B+1188A/C and Susceptibility of Coal Worker's Pneumoconiosis in Han Ethnic Group in Ningxia Area[J]. Journal of Environmental and Occupational Medicine, 2011, 28(9): 535-538.
Citation: DANG Zhan-cui , SONG Qi-ru , LI Wu-ping , YANG Wen-hai , WU Xiang-lin . Gene Polymorphisms of Interleukin-12B+1188A/C and Susceptibility of Coal Worker's Pneumoconiosis in Han Ethnic Group in Ningxia Area[J]. Journal of Environmental and Occupational Medicine, 2011, 28(9): 535-538.

IL-12B+1188A/C基因多态性与宁夏汉族煤工尘肺易感性研究

Gene Polymorphisms of Interleukin-12B+1188A/C and Susceptibility of Coal Worker's Pneumoconiosis in Han Ethnic Group in Ningxia Area

  • 摘要: 目的 探讨白介素-12B(interleukin-12B, IL-12B)基因3'UTR区1188A/C位点多态性与宁夏汉族煤工尘肺(coal worker's pneumoconiosis, CWP)易感性的关系。

    方法 采用聚合酶链反应-限制片段长度多态性(PCR-RFLP)法对120例CWP患者和120例煤尘接触组IL-12B基因1188A/C位点多态性进行分型,对等位基因及基因型分布进行统计学分析。

    结果 煤工尘肺组与煤尘接触组在接尘工龄、工种构成、吸烟烟龄差异无统计学意义(P>0.05)。对年龄进行标化后,分析发现IL-12B 1188A/C位点在煤工尘肺组和煤尘接触组中检出三种基因型AA、AC、CC型,这三种基因型在尘肺组和接触组间分布差异无统计学意义(P>0.05);等位基因C在煤工尘肺组和煤尘接触组中所占比例分别为45.8%和36.7%,差异有统计学意义(P<0.05),在接尘工龄、工种和是否吸烟中的分布无差异(P>0.05)。

    结论 IL-12B 1188A/C位点C等位基因可能是宁夏汉族煤工尘肺的易感基因之一。

     

    Abstract: Objective To investigate gene polymorphisms of Interle ukin-12B (IL-12B+1188A/C) and susceptibility of coal worker's pneumoconiosis (CWP) in Han ethnic group workers.

    Methods SNP 1188A/C of IL-12B gene was detected by polymerase chain reaction (PCR) based on restriction fragment length polymorphism (RFLP) technique in 120 CWP patients and 120 coal dust exposure workers.

    Results The length of exposure to dust,job title,age of smoking frequencies were not significantly different between the cases and the controls (P>0.05).Three genotypes (AA,AC,CC) were detected in IL-12B (1188A/C) and no significant difference was found between the CWP group and the controls (P>0.05) after age standardization.The proportion of C allele showed statistical difference between the cases (45.8%) and the controls (36.7%).Significant difference between the CWP patients and the controls was found in frequencies of C alleles (P<0.05).The distributions in the dust exposure duration,job title and smoking were no significant differences (P>0.05).

    Conclusion The study found that IL-12B+1188A/C sites C allele may be one of the susceptible gene in CWP of Han ethnic group in Ningxia area.

     

/

返回文章
返回