李冰, 李昕, 朱博, 张新玉, 刘丹, 邢晓越, 王欣, 张琪, 孙贵范. 无机砷对Chang肝细胞株血红素单加氧酶-1 mRNA和蛋白表达的诱导作用[J]. 环境与职业医学, 2011, 28(8): 471-474.
引用本文: 李冰, 李昕, 朱博, 张新玉, 刘丹, 邢晓越, 王欣, 张琪, 孙贵范. 无机砷对Chang肝细胞株血红素单加氧酶-1 mRNA和蛋白表达的诱导作用[J]. 环境与职业医学, 2011, 28(8): 471-474.
LI Bing , LI Xin , ZHU Bo , ZHANG Xin-yu , LIU Dan , XING Xiao-yue , WANG Xin , ZHANG Qi , SUN Gui-fan . Effects of Inorganic Arsenic on mRNA and Protein Expression of Heme Oxygenase-1 in Chang Hepatocytes[J]. Journal of Environmental and Occupational Medicine, 2011, 28(8): 471-474.
Citation: LI Bing , LI Xin , ZHU Bo , ZHANG Xin-yu , LIU Dan , XING Xiao-yue , WANG Xin , ZHANG Qi , SUN Gui-fan . Effects of Inorganic Arsenic on mRNA and Protein Expression of Heme Oxygenase-1 in Chang Hepatocytes[J]. Journal of Environmental and Occupational Medicine, 2011, 28(8): 471-474.

无机砷对Chang肝细胞株血红素单加氧酶-1 mRNA和蛋白表达的诱导作用

Effects of Inorganic Arsenic on mRNA and Protein Expression of Heme Oxygenase-1 in Chang Hepatocytes

  • 摘要: 目的 观察亚砷酸钠(sodium arsenite, NaASO2)对Chang肝细胞株血红素单加氧酶-1(heme oxygenase-1,HO-1)mRNA和蛋白表达的诱导作用。

    方法 以5μmol/L和10μmol/L的NaASO2作用Chang肝细胞株2、6、12h和24h,分别用逆转录-聚合酶链反应(RT-PCR)和Western Blot法检测细胞内HO-1的mRNA和蛋白表达情况。

    结果 5 μmol/L和10μmol/L NaASO2暴露2 h开始出现HO-1 mRNA的诱导表达; 6h的表达水平明显高于对照组和2h暴露组(P<0.01)。其中, 10 μmol/L NaASO2暴露12 h和24 h的mRNA表达均明显高于该浓度的6 h暴露组(P<0.01);但24 h的HO-1 mRNA表达水平与12 h组相比没有明显升高。NaASO2暴露诱导的HO-1蛋白表达则从6 h开始出现, 12 h组明显高于6 h组, 24 h组明显高于12 h组(均P<0.01); 5 μmol/L和10 μmol/L NaASO2分别暴露6、12 h和24 h的HO-1蛋白表达量分别是对照组的2.80、9.34、18.15和3.97、12.92、23.29倍;此外, 10 μmol/L NaASO2暴露12 h和24 h的HO-1 mRNA和蛋白表达均明显高于对应时间的5 μmol/L组(P<0.01)。

    结论 NaASO2暴露能够有效和持续性诱导Chang肝细胞株HO-1的mRNA和蛋白表达,是无机砷暴露的一种细胞保护性适应性反应。

     

    Abstract: Objective To observe the effects of inorganic arsenic on mRNA and protein expression of heme oxygenase-1(HO-1) in Chang hepatocytes.

    Methods Sodium arsenite (NaASO2,5 and 10μmol/L) were given to the Chang hepatocytes for 2,6,12 and 24 h,then reverse transcriptase-polymerase chain reaction (RT-PCR) and Western Blot were used to evaluate the expression of HO-1 mRNA and protein.

    Results The 5 and 10 μmol/L NaASO2 induced HO-1 mRNA expression after 2 h.The expression increased notably at 6 h (P<0.01).The 10 μmol/L NaASO2-induced HO-1 mRNA expression was significantly higher at both 12 h and 24h than at 6h exposure (P<0.01).Even at 24h,the HO-1 mRNA expression level was still at a steady value similar to that of 12h.In contrast,the 5 μmol/L and 10 μmol/L NaASO2 induced HO-1 protein expression could be observed from 6 h (2.80 and 3.97 folds to the control),continued to increase at 12 h (9.34 and 12.92 folds),and further up-regulated at 24 h (18.15 and 23.29 folds)(P<0.01).Furthermore,the 10 μmol/L NaASO2 treatment induced a remarkably higher HO-1 mRNA and protein expression than the 5 μmol/L exposure at the corresponding exposure times (P<0.01) of 12 h and 24 h.

    Conclusion NaASO2 can induce a strong and sustained HO-1 mRNA and protein expression in Chang hepatocytes,which suggest to be protective adaptive changes of hepatocytes in response to inorganic arsenic exposure.

     

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