郭庆峰, 孙建娅. 职业性镍接触工人DNA链断裂的研究[J]. 环境与职业医学, 2011, 28(7): 426-429.
引用本文: 郭庆峰, 孙建娅. 职业性镍接触工人DNA链断裂的研究[J]. 环境与职业医学, 2011, 28(7): 426-429.
GUO Qing-feng , SUN Jian-ya . Study on DNA Strand Breaks in Stainless Steel Workers Exposed to Nickel[J]. Journal of Environmental and Occupational Medicine, 2011, 28(7): 426-429.
Citation: GUO Qing-feng , SUN Jian-ya . Study on DNA Strand Breaks in Stainless Steel Workers Exposed to Nickel[J]. Journal of Environmental and Occupational Medicine, 2011, 28(7): 426-429.

职业性镍接触工人DNA链断裂的研究

Study on DNA Strand Breaks in Stainless Steel Workers Exposed to Nickel

  • 摘要: 目的 观察不锈钢冶炼职业性镍接触对工人淋巴细胞DNA损伤的影响。

    方法 对某不锈钢冶炼厂相关作业环境进行监测,根据作业工种的不同,选取该厂镍接触的炼钢工(60名)、钢渣处理工(65名)及轧钢工(40名),共165名男性工人为接触组,同时选取该企业67名水处理工为对照组,通过问卷调查收集研究对象的一般情况、职业暴露、吸烟和饮酒情况等,应用彗星试验检测工人外周血淋巴细胞的DNA损伤。

    结果 炼钢工的尾惯量为(23.33& #177;7.10)%,钢渣处理工为(22.87& #177;10.67)%;轧钢工为(21.67& #177;9.32)%, 3组均高于对照组(10.53& #177;5.08)%(P<0.01);炼钢工的尾长为(1.20& #177;0.21)μm,钢渣处理工为(1.18& #177;0.22)μm,轧钢工为(1.14& #177;0.24)μm, 3组也均高于对照组(0.91& #177;0.19)μm(P<0.01);炼钢工、钢渣处理工和的轧钢工Olive尾矩(olive tail moment, OTM)分别为0.495& #177;0.055、0.455& #177;0.045和0.429& #177;0.069,均高于对照组(0.103& #177;0.054) (P<0.01)。各工龄组之间OTM差异均有统计学意义(P<0.01);吸烟与非吸烟组之间比较, OTM值差异也有统计学意义(P<0.05)。经相关分析表明:OTM与尿镍浓度呈正相关(r=0.576,P<0.01)。

    结论 淋巴细胞DNA损伤可能是镍职业接触工人的早期损害之一。

     

    Abstract: Objective To explore DNA damage of peripheral lymphocyte of wokers exposed to nickel in a stainless steel refining factory.

    Methods Occupational hazardous factors in a stainless steel factory were monitored, a total of 165 nickel exposed workers were recruited, including 60 steel refining workers, 65 slaggers and 40 millmen. In addition, 67 workers in water treatment department not exposured to nickel were selected and matched with those exposed workers by age and cigarette smoking. Comet assay was conducted for DNA damage of lymphocyte isolated from peripheral blood samples of all participating subjects.

    Results The comet assay indicated that the exposed workers had greater DNA damage reflected by tail inertia, tail length and olive tail moment (OTM) all different from control subjects (P < 0.01). Tail inertia of the steel refining workers (23.33& #177;7.10) and of the slaggers (22.87& #177;10.67)were obviously higher than the contro(l 10.53& #177;5.08) (P < 0.01). Tail inertia of the millmen (21.67& #177;9.32) was also higher than the control (P < 0.05). Tail length of the steel refining workers (1.20& #177;0.21), of the slaggers (1.18& #177;0.22) and of the millmen (1.14& #177;0.24) were obviously higher than the control (0.91& #177;0.19) too. The OTM of the steel refining workers (0.495& #177;0.055), of the slaggers (0.455& #177;0.045) and of the millmen (0.429& #177;0.069) were all significantly higher than the control (P < 0.01). Significant differences were showed in the OTM values between non-smoking and smoking workers. Correlation analysis showed that there was a significant positive correlation between the urine concentration of nickel and the OTM (r=0.576, P < 0.001).

    Conclusion DNA strand break in lymphocytes should be an earlier impairment of occupational nickel-exposed population. Comet assay may be used for early monitoring of occupational nickel-exposed population.

     

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