周连芳, 寿卫国, 吴国华, 薛振宇. 微核试验和彗星试验观察职业性二甲基乙酰胺暴露的遗传损伤[J]. 环境与职业医学, 2011, 28(2): 98-99.
引用本文: 周连芳, 寿卫国, 吴国华, 薛振宇. 微核试验和彗星试验观察职业性二甲基乙酰胺暴露的遗传损伤[J]. 环境与职业医学, 2011, 28(2): 98-99.
ZHOU Lian-fang , SHOU Wei-guo , WU Guo-hua , XUE Zhen-yu . Detection of Genetic Damages of Occupational Dimethylacetamide Exposure with Micronucleus Test and Comet Assay[J]. Journal of Environmental and Occupational Medicine, 2011, 28(2): 98-99.
Citation: ZHOU Lian-fang , SHOU Wei-guo , WU Guo-hua , XUE Zhen-yu . Detection of Genetic Damages of Occupational Dimethylacetamide Exposure with Micronucleus Test and Comet Assay[J]. Journal of Environmental and Occupational Medicine, 2011, 28(2): 98-99.

微核试验和彗星试验观察职业性二甲基乙酰胺暴露的遗传损伤

Detection of Genetic Damages of Occupational Dimethylacetamide Exposure with Micronucleus Test and Comet Assay

  • 摘要: 目的 观察二甲基乙酰胺(DMAc)对职业接触人群所产生的遗传损伤。

    方法 用胞质阻断微核试验(CBMN)和彗星试验(SCGE)检测某氨纶企业DMAc接触工人(30名)及非接触DMAc的对照人员(30名)的外周血淋巴细胞DNA和染色体损伤。

    结论 接触组微核率、微核细胞率、核分裂指数分别为(4.67& #177;2.25)‰、(4.56& #177;2.19)‰、(1.82& #177;0.30)‰,对照组分别为(5.00& #177;2.62)‰、(4.89& #177;2.59)‰、(1.73& #177;0.25)‰,两组的各指标间差异均无统计学意义(P>0.05)。接触组彗星尾DNA百分比、细胞尾长、尾相分别为(4.76& #177;2.63)%、(12.60& #177;5.68)μm、(2.51& #177;2.30),对照组分别为(4.49& #177;2.48)%、(11.77& #177;5.01)μm、(2.28& #177;1.89),各指标间的差异均无统计学意义(P>0.05)。

    结论 在本实验条件下,职业性DMAc接触人群外周血淋巴细胞的染色体和DNA未表现出遗传损伤。

     

    Abstract: Objective To study the genetic damages of occupational exposure to Dimethylacetamide (DMAc).

    Methods The damages of DNA and chromosomes of peripheral blood lymphocytes were determined by comet assay and cytokinesis-block micronucleus test in exposed group consisting of 30 workers occupationally exposed to DMAc and a control group consisting of 30 workers without exposure to DMAc.

    Results In the exposure group, the micronucleus rate, micronucleus cell rate, and nuclei division index were (4.67& #177;2.25)‰(, 4.56& #177;2.19)‰(, 1.82& #177;0.30)‰, and showed no statistical significance of difference between those versus to the control group(5.00& #177;2.62)‰, (4.89& #177;2.59)‰, (1.73& #177;0.25)‰ (P>0.05). The Tail DNA%, mean tail length, and meadians of mean tail moment were(4.76& #177;2.63)%,(12.60& #177;5.68) μm,(2.51& #177;2.30), and showed no statistical significance difference between those to the control group(4.49& #177;2.48)%,(11.77& #177;5.01) μm,(2.28& #177;1.89) (P>0.05).

    Conclusion Under condition in this experiment, DMAc occupational exposure exhibited no damage effect on DNA and chromosomes on workers.

     

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