妊娠早期接触丙烯腈对子代大鼠心肌缺血再灌注损伤的影响
Effects of Acrylonitrile Exposure in First Trimester on Myocardial Ischemia-Reperfusion Injury in Rat Offsprings
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摘要:
目的 评价大鼠妊娠早期接触丙烯腈对子代成年后心肌缺血再灌注损伤敏感度的影响。 方法 2月龄雄性大鼠5只和雌性大鼠12只随机分组,对照组及丙烯腈低、中、高浓度组,合笼,确认雌性大鼠怀孕后于孕期第1~7天给予不同浓度丙烯腈染毒,仔鼠正常条件下饲养至3个月作为实验动物;使用Langendorff系统分别记录心脏复跳后和缺血60 min再灌注后各组仔鼠心脏心率(heart rate,HR)、冠脉流量(coronary flow,CF)、左室舒张末压(left ventricular end-diastolic pressure,LVEDP)、左室收缩压(left ventricular systolic pressure,LVSP)、最大压力导数(dp/dt)max和最小压力导数(dp/dt)min,计算梗死心肌占总心脏重量的比例。 结果 对照组和丙烯腈染毒组心脏复跳后基础心功能、冠脉流量、心率差异无统计学意义(P>0.05);但缺血再灌注后丙烯腈染毒组上述指标明显低于对照组(P<0.05,P<0.01),而且中浓度丙烯腈染毒组上述指标低于低浓度染毒组(P<0.05,P<0.01)。丙烯腈染毒组梗死心肌重量占总心脏重量比例明显大于对照组(P<0.05)。 结论 大鼠妊娠早期接触丙烯腈对子代成年后心肌缺血再灌注损伤敏感度增加;而且接触丙烯腈较高浓度者其损伤敏感度亦增加,诱发心律失常比例升高。 Abstract:Objective To evaluate the effects of acrylonitrile exposure in first trimester on myocardial ischemiareperfusion injury in adult rat offsprings. Methods Sprague-Dawley rats (5 males and 12 females) were randomized into control group, and low, mid and high acrylonitrile groups. After mating and confirming pregnancy, female rats were exposed to acrylonitrile in different concentrations in first trimester (from day 1 to 7). The male offsprings were raised in normal condition. At age of 3 months, the hearts of offsprings were isolated and perfused in a Langendorff apparatus. Data were recorded after heart returning to beat and 60 min of myocardial ischemia followed by reperfusion respectively, including heart rate (HR), coronary flow (CF), left ventricular end-diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP), (dp/dt)max, (dp/dt)min and the left ventricular (LV) in farct size. Results The basal heart functional parameters, heart rate and coronary flow were not different between the control group and the acrylonitrile-exposure groups after returning to heart beat (P>0.05). But after ischemia-reperfusion injury, the data were significantly lower in the acrylonitrile groups than in the control group (P<0.05, P<0.01), especially in the mid dose group (P<0.05, P<0.01). The weight proportion of myocardial infarction to whole heart was larger in the acrylonitrile groups (P<0.05). Conclusion Acrylonitrile exposure in first trimester increases susceptibility to myocardial ischemia-reperfusion injury in the offsprings. Moreover, heart function worsening and arrhythmogenesis increasing could be resulted from the maternal rats exposed to higher level of acrylonitrile.
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