熊丽蓓, 姜觅, 戴承兵. 高效液相色谱法测定密胺容器中三聚氰胺迁移量的研究[J]. 环境与职业医学, 2011, 28(10): 594-597.
引用本文: 熊丽蓓, 姜觅, 戴承兵. 高效液相色谱法测定密胺容器中三聚氰胺迁移量的研究[J]. 环境与职业医学, 2011, 28(10): 594-597.
XIONG Li-bei , JIANG Mi , DAI Cheng-bing . Study on the Method for Determining Migration of Melamine from Melamine-Formaldehyde Containers by HPLC[J]. Journal of Environmental and Occupational Medicine, 2011, 28(10): 594-597.
Citation: XIONG Li-bei , JIANG Mi , DAI Cheng-bing . Study on the Method for Determining Migration of Melamine from Melamine-Formaldehyde Containers by HPLC[J]. Journal of Environmental and Occupational Medicine, 2011, 28(10): 594-597.

高效液相色谱法测定密胺容器中三聚氰胺迁移量的研究

Study on the Method for Determining Migration of Melamine from Melamine-Formaldehyde Containers by HPLC

  • 摘要: 目的 采用4种溶剂浸泡提取,建立一个快速、简便的三聚氰胺-甲醛食品容器中三聚氰胺迁移量的高效液相色谱(HPLC)测定方法。

    方法 三聚氰胺-甲醛(密胺)食品容器采用水、4%乙酸、15%乙醇和正己烷4种溶剂浸泡提取,其浸泡液过滤至高效液相色谱仪,经反相色谱分离后,由紫外检测器检测分离、测定,外标法定量。

    结果 从色谱条件和样品浸泡液的选择等各方面研究了三聚氰胺-甲醛食品容器中三聚氰胺迁移量的分析方法,该方法的定量限10 μg/dm2,方法的线性范围为0.050~2.000 μg/mL。样品加标回收范围为85.7%~105.7%,样品4种浸泡液测定结果的相对标准偏差为0.43%~4.80%。

    结论 本方法是一个测定三聚氰胺-甲醛食品容器中三聚氰胺迁移量快速、有效、稳定性好、灵敏度高的测定方法。

     

    Abstract: Objective To develop a rapid and simple high-performance liquid chromatography (HPLC) method for determining the migration of melamine extracted by four solvents from melamine-formaldehyde products used as food containers.

    Methods Melamine were soaked out by four solvents which were water, 4% acetic acid, 15% ethanol and n-hexane. The four soaks were separated by reversed phase chromatographic column and detected by UV-detector.

    Results The analysis method of determining the migration of melamine from melamine-formaldehyde food containers was studied from the aspects of chromatogram and soaking solutions. The detection limit was 10μg/dm2. The linear range was between 0.050μg/mL and 2.000μg/mL. The recovery was controlled between 85.7% and 105.7%. The relative standard deviation was between 0.43% and 4.80%.

    Conclusion This method was rapid, effective, stabilize and sensitive for detecting the migration of melamine from melamine-formaldehyde products used as food containers.

     

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