微波预先辐照致γ射线对小鼠骨髓基质细胞毒性作用的改变
Pre-exposure with Microwave Alters the Effects of γ-rays on Mouse Bone Marrow Stromal Cells
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摘要:
目的 探讨微波预先辐照对γ射线致骨髓基质细胞凋亡、线粒体膜电位、胞内游离Ca2+浓度和Ca2+-Mg2+-ATP酶活性改变的影响。 方法 将原代培养的骨髓基质细胞随机分为正常对照组、单纯微波组、单纯γ射线组和联合组。12 μW/cm2微波对单纯微波组和联合组连续辐照7 d,每天1 h,第8天对单纯γ射线组和联合组进行5 Gy的γ射线照射。用钙离子依赖性磷脂结合蛋白-异硫氰酸荧光素及碘化丙啶(Annexin V-FITC及PI)双标记法检测细胞凋亡,流式细胞仪检测线粒体膜电位和胞内游离Ca2+浓度,试剂盒检测Ca2+-Mg2+-ATP酶活性。 结果 与正常对照组相比,各处理组细胞的凋亡率和线粒体膜电位未见明显改变。γ射线照射后24 h,单纯微波组、联合组和单纯γ射线组细胞内游离Ca2+浓度明显升高(P<0.05),Ca2+-Mg2+-ATP酶活性明显降低(P<0.05)。与单纯γ射线组相比,联合组细胞内游离Ca2+浓度明显降低(P < 0.05),Ca2+-Mg2+-ATP酶活性明显升高(P < 0.05)。 结论 本实验条件下,900 MHz,12 μW/cm2的微波辐射和5Gy γ射线均不能引起细胞凋亡率、线粒体膜电位的明显变化,但能导致胞内游离Ca2+浓度明显上升和Ca2+-Mg2+-ATP酶活性明显降低。预先微波辐照能够明显减弱γ射线引起的胞内游离Ca2+浓度和Ca2+-Mg2+-ATP酶活性改变。 Abstract:Objective To explore the effects of pre-exposure with microwave on γ-rays induced apoptosis, mitochondrion membrane potential (MMP), intracellular Ca2+ concentration and Ca2+-Mg2+-ATPase activity in mouse bone marrow stromal cells. Methods Primarily cultured mouse bone marrow stromal cells were randomly divided into control group, microwave alone group, γ-rays group and combined exposure group. The microwave alone and combined exposure groups were exposed to 12 μW/cm2 microwave 1 h a day for 7 days. The γ-rays alone and combined exposure groups were subsequently exposed to 5 Gy 60Co γ-rays on the 8th day. Ca2+ concentration and MMP were detected by a flow cytometry (FCM). Apoptosis was detected by Annexin V-FITC/PI, and Ca2+-Mg2+-ATPase activities were analyzed by a commercial kit. Results There were no significantly differences between different groups in apoptosis and MMP. 24 h after γ-rays irradiation, the Ca2+ concentrations of the microwave alone, the γ-rays alone and the combined exposure groups were statistically higher than those of the control group (P < 0.05), while Ca2+-Mg2+-ATPase activities were statistically lower than those of the control group (P<0.05). Compared with the γ-rays alone group, Ca2+ concentrations of the combined exposure group significantly decreased (P < 0.05)and Ca2+-Mg2+-ATPase activity statistically increased (P < 0.05). Conclusion Under the experimental condition, microwave radiation (900 MHz, 12 μW/cm2)and 5 Gy γ-rays did not induce evident changes in apoptosis and MMP; but did induce an increase of Ca2+ concentrations and a decrease of Ca2+-Mg2+-ATPase activity. Pre-exposure with microwave could significantly decrease γ-rays induced changes in Ca2+ concentration and Ca2+-Mg2+-ATPase activity.
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