谭红汕, 王琪, 王爱红, 冯楠楠, 叶云杰, 冯晓青, 郑玉新, 夏昭林. 1,3-丁二烯致染色体损伤的遗传易感性与CYP2E1GSTs基因多态性关系[J]. 环境与职业医学, 2010, 27(5): 266-269.
引用本文: 谭红汕, 王琪, 王爱红, 冯楠楠, 叶云杰, 冯晓青, 郑玉新, 夏昭林. 1,3-丁二烯致染色体损伤的遗传易感性与CYP2E1GSTs基因多态性关系[J]. 环境与职业医学, 2010, 27(5): 266-269.
TAN Hong-shan , WANG Qi , WANG Ai-hong , FENG Nannan , YE Yun-jie , FENG Xiao-qing , ZHENG Yu-xin , XIA Zhao-lin . Study on the Relationship between Chromosomal Damage and Polymorphisms of Metabolizing Enzymes in 1,3-butadiene-exposed Workers[J]. Journal of Environmental and Occupational Medicine, 2010, 27(5): 266-269.
Citation: TAN Hong-shan , WANG Qi , WANG Ai-hong , FENG Nannan , YE Yun-jie , FENG Xiao-qing , ZHENG Yu-xin , XIA Zhao-lin . Study on the Relationship between Chromosomal Damage and Polymorphisms of Metabolizing Enzymes in 1,3-butadiene-exposed Workers[J]. Journal of Environmental and Occupational Medicine, 2010, 27(5): 266-269.

1,3-丁二烯致染色体损伤的遗传易感性与CYP2E1GSTs基因多态性关系

Study on the Relationship between Chromosomal Damage and Polymorphisms of Metabolizing Enzymes in 1,3-butadiene-exposed Workers

  • 摘要: 目的 探讨代谢酶基因CYP2E1GSTs的基因多态性与1,3-丁二烯致外周血淋巴细胞染色体损伤易感性的关系。

    方法 采用胞质分裂阻滞微核试验方法(CBMN)评价166名丁二烯接触工人和41名对照组染色体损伤水平,应用PCR-RFLP测CYP2E1 c1c2基因多态, PCR法测GSTT1GSTM1缺失情况。

    结果 接触组和对照组的微核发生率分别为(3.23& #177;2.49)‰和(1.22& #177;1.19)‰,差别有统计学意义(P<0.01)。多因素Poisson回归分析发现CYP2E1 c1c2/c2c2基因型和GSTM1非缺失型与染色体损伤相关(分别为χ2=14.39, P<0.01和χ2=4.23, P<0.05)。没有发现年龄、工龄、性别、吸烟和饮酒等与微核率之间的关系。

    结论 双核淋巴细胞微核数可以作为1,3-丁二烯接触早期健康损害的指标。CYP2E1 c1c2/c2c2基因型、GSTM1非缺失型与1,3-丁二烯诱导的染色体损伤有关。

     

    Abstract: Objective To explore the relationship between chromosomal damage induced by 1,3-butadiene(BD) and polymorphisms of metabolizing enzymes of CYP2E1 and GSTs.

    Methods Cytokinesis-block micronucleus(CBMN) assay was used to detect chromosomal damage in peripheral lymphocytes of 166 1,3-butadiene exposed workers and 41 controls. PCRRFLP technique was applied to detect polymorphisms in CYP2E1 c1c2. Null genotypes of GSTT1 and GSTM1 were detected by PCR.

    Results Compared the CBMN frequencies of the two groups we found that the frequency in exposed workers was higher than that in control group (P<0.01). Individuals with the CYP2E1 c1c2/c2c2 and GSTM1 positive genotype were observed to have significantly higher CBMN frequencies than individuals with more common genotypes (χ2=14.39, P<0.01 and χ2=4.23, P<0.05). Sex, age, duration of work, smoking and drinking habit had no relationship with CBMN frequencies.

    Conclusion BD-exposed workers had higher risk of chromosomal damage compared with control. Cytokinesis-block micronucleus(CBMN) assay can be used to evaluate the early damage of butadiene-exposed workers. CYP2E1 c1c2/c2c2 and GSTM1 positive genotype showed higher average CBMN frequencies than those with more common genotypes.

     

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