细颗粒物暴露对人群DNA损伤的影响
DNA Damage in Population Exposed to Fine Particulate
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摘要:
目的 研究人群PM2.5暴露对外周血淋巴细胞DNA的损伤作用。 方法 选择不吸烟或戒烟半年以上且家庭中也无人吸烟的男性交通警察107人为高暴露组,普通社区男性居民101人为低暴露组。使用个体采样器24 h连续监测两组的PM2.5暴露情况,并采集血液进行淋巴细胞彗星试验(单细胞凝胶电泳试验),以判断两组的DNA损伤情况。 结果 高暴露组PM2.5日平均暴露浓度为(115.40& #177;46.17)μg/m3,高于低暴露组的(74.94& #177;40.09)μg/m3(P < 0.01)。高暴露组彗尾率(15.20& #177;3.46)%和Olive尾矩1.25& #177;0.29高于低暴露组的(10.05& #177;3.45)%和0.86& #177;0.22(P < 0.01)。DNA损伤程度随着PM2.5日均暴露量增大而增加(OR=1.032, P < 0.01),未发现年龄与工龄对DNA损伤程度的明显影响。 结论 在正常大气暴露下, PM2.5浓度升高能导致人群DNA损伤程度增加。 Abstract:Objective To study DNA damage in peripheral blood lymphocyte induced by PM2.5. Methods One hundred and seven traffic policemen were selected as exposed population and one hundred and one common residents were selected as the control. All members were male nonsmokers or ones who had quitted smoking for at least 6 months and so were their family members, all of them were between 25 and 55 years old. The level of exposure to PM2.5 was obtained by personal monitor of PM2.5 for 24 h. Blood samples were then collected to detect DNA damage by single cell gelelectrophoresis assay. Results The average level of exposure to PM2.5 per day in exposed group(115.40& #177;46.17) μg/m3 was significantly higher than that in control group (74.94& #177;40.09)μg/m3. Comet cell percentage was (15.20& #177;3.46)% and Olive moment was 1.25& #177;0.29 in exposed group, which were both significantly higher than those in control group (10.05& #177;3.45)% and 0.86& #177;0.22. The level of DNA damage became higher as the exposure level increased, but no significant correlation with age or length of service was found. Conclusion Exposed to higher level of airborne PM2.5 could result in higher DNA damage.
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