金辉, 徐小敏, 糜祖煌, 刘沛. 鲍曼不动杆菌的耐药基因分型与脉冲场凝胶电泳分型的比较[J]. 环境与职业医学, 2010, 27(4): 219-221,225.
引用本文: 金辉, 徐小敏, 糜祖煌, 刘沛. 鲍曼不动杆菌的耐药基因分型与脉冲场凝胶电泳分型的比较[J]. 环境与职业医学, 2010, 27(4): 219-221,225.
JIN Hui , XU Xiao-min , MI Zu-huang , LIU Pei . Comparison of Drug Resistant Genes Typing and Pulsed-field Gel Electrophoresis Typing for Acinetobacter Baumannii[J]. Journal of Environmental and Occupational Medicine, 2010, 27(4): 219-221,225.
Citation: JIN Hui , XU Xiao-min , MI Zu-huang , LIU Pei . Comparison of Drug Resistant Genes Typing and Pulsed-field Gel Electrophoresis Typing for Acinetobacter Baumannii[J]. Journal of Environmental and Occupational Medicine, 2010, 27(4): 219-221,225.

鲍曼不动杆菌的耐药基因分型与脉冲场凝胶电泳分型的比较

Comparison of Drug Resistant Genes Typing and Pulsed-field Gel Electrophoresis Typing for Acinetobacter Baumannii

  • 摘要: 目的 了解耐药鲍曼不动杆菌(Acinetobacter baumannii, Ab)中耐药基因的分布情况, 并比较基于耐药基因分型和脉冲场凝胶电泳分型的差异性。

    方法 对 2005年宁波市第二医院收集的 27株流行和散发的 Ab进行耐药分析, 并对脉冲场凝胶电泳方法和基于耐药基因聚合酶链反应的聚类分析方法进行比较。

    结果 27株 Ab耐多种抗生素和携带多种耐药基因。基于多个类别的耐药基因分型与脉冲场凝胶电泳方法分型相比有 88.9%的一致性, 而且耐药基因分型能更好地反映不同耐药株的耐药过程。

    结论 与脉冲场凝胶电泳方法相比, 耐药基因分型方法可以用于院内感染的流行研究, 且有助于临床的诊断和治疗。

     

    Abstract: Objective To study the epidemiology of drug resistant genes of Acinetobacter baumannii and to compare the difference between the two genotyping methods, i.e. drug resistant gene typing(DRGT)and pulsed-field gel electrophoresis(PFGE).

    Methods Twenty-seven isolates of Acinetobacter baumannii were collected in the Second Hospital of Ningbo during 2005, including both epidemic and sporadic events. Susceptibility test, PFGE, DRGT and cluster analysis were carried out to confirm the drug resistance and to analyze the genotyping, respectively.

    Results Twenty-seven strains of Acinetobacter displayed multiple antibiotic resistance and drug resistant genes. The result of DRGT based on various drug resistant genes was comparable to PFGE in Acinetobacter strains with the concordance of 88.9%, and even suggested a molecular evolution course of different drug-resistant strains.

    Conclusion Compared to PFGE, DRGT is useful to analyze molecular epidemiology of nosocomial infections, which would benefits clinical diagnosis and therapy.

     

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