林强, 朱洪, 吴丹梅, 谈顺, 张毅. 双酚A对人早孕绒毛组织结构及β-HCG分泌功能的影响[J]. 环境与职业医学, 2010, 27(4): 216-218.
引用本文: 林强, 朱洪, 吴丹梅, 谈顺, 张毅. 双酚A对人早孕绒毛组织结构及β-HCG分泌功能的影响[J]. 环境与职业医学, 2010, 27(4): 216-218.
LIN Qiang , ZHU Hong , WU Dan-mei , TAN Shun , ZHANG Yi . Study on the Effects of Bisphenol A on the Morphology and β-HCG Secretion of Human Early Villus[J]. Journal of Environmental and Occupational Medicine, 2010, 27(4): 216-218.
Citation: LIN Qiang , ZHU Hong , WU Dan-mei , TAN Shun , ZHANG Yi . Study on the Effects of Bisphenol A on the Morphology and β-HCG Secretion of Human Early Villus[J]. Journal of Environmental and Occupational Medicine, 2010, 27(4): 216-218.

双酚A对人早孕绒毛组织结构及β-HCG分泌功能的影响

Study on the Effects of Bisphenol A on the Morphology and β-HCG Secretion of Human Early Villus

  • 摘要: 目的 研究双酚 A(BPA)水平与人早孕绒毛膜促性腺激素(β-HCG)分泌功能的关系, 探讨 BPA对绒毛组织可能带来的影响。

    方法 以 40例早期妊娠(6~8周)的新鲜绒毛组织进行体外培养, 培养基中加入不同浓度的BPA:A组为 1000& #215;10-6 g/L, B组为 500& #215;10-6 g/L, C组为 1& #215;10-6 g/L, D组为对照组。取第 2、4天的培养液测定 β-HCG;第 4天将培养的绒毛组织作病理检查。

    结果 ①随培养时间增加, D组的 β-HCG浓度呈递减变化,而其他组呈递增变化,且差异有统计学意义(P=0.001); 随 BPA浓度的增加, β-HCG的浓度也逐渐增加, 各组间第 4天与第 2天 β-HCG的差异有统计学意义(P=0.002)。② D组绒毛结构正常, 其他组绒毛合体滋养细胞明显增生, 结节数量有不同程度增多, 且出现绒毛间质水肿、纤维素样坏死、退行性变化, 部分绒毛出现萎缩。

    结论 BPA对绒毛组织具有细胞毒性作用, 并可影响滋养细胞的分泌功能。

     

    Abstract: Objective To study the effects of bisphenol A(BPA)on the morphology and β-HCG secretion of human early villus and the posible toxicity of BPA to human early villus

    Methods The human early villus from forty preganancy cases(6-8 weeks)were collected, cultured and treated with BPA at different concentrations. The cultures were divided into 4 groups based on the concentration of BPA. The concentration of BPA was 1 000& #215;10-6 g/L in group A,500& #215;10-6 g/L in group B, 1& #215;10-6 g/L in group C respectively, and without BPA in group D as control. The concentration of β-HCG in the supernatant of culture was measured by chemiluminescence immunoassay at day 2 and day 4 of culture, and the cultured villus was stained by HE at day 4.

    Results ① The concentration of β-HCG in the culture was elevated in A, B and C group with increasing culture time, while its concentration in D group was decreased. The concentration of β-HCG was statistically higher in group A, B and C than in group D. The concentration of β-HCG was increased with the increasing BPA.The differences of β-HCG values between day 4 and day 2 among the groups were statistically different(P=0.002).② The histologic abnormalities of villus were characterized by increasing syncytiotrophoblast knotting, prominent cytotrophoblast proliferating, basement membrane thickening, fibrinoid necrosis and anaplasia. In addition, atrophy was also observed in some villus.

    Conclusion In culture, BPA is cytotoxic to villus and has effects on the secretion of trophocyte.

     

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