曹培, 田金凤, 杨新军, 闫洪涛. 全氟辛酸和全氟辛烷磺酸经大鼠离体皮肤吸收实验[J]. 环境与职业医学, 2010, 27(3): 150-152.
引用本文: 曹培, 田金凤, 杨新军, 闫洪涛. 全氟辛酸和全氟辛烷磺酸经大鼠离体皮肤吸收实验[J]. 环境与职业医学, 2010, 27(3): 150-152.
CAO Pei , TIAN Jin-feng , YANG Xin-jun , YAN Hong-tao . In Vitro Experiment on Percutaneous Absorption of Perfluorooctanoate (PFOA) and Perfluorooctane Sulfonate (PFOS)[J]. Journal of Environmental and Occupational Medicine, 2010, 27(3): 150-152.
Citation: CAO Pei , TIAN Jin-feng , YANG Xin-jun , YAN Hong-tao . In Vitro Experiment on Percutaneous Absorption of Perfluorooctanoate (PFOA) and Perfluorooctane Sulfonate (PFOS)[J]. Journal of Environmental and Occupational Medicine, 2010, 27(3): 150-152.

全氟辛酸和全氟辛烷磺酸经大鼠离体皮肤吸收实验

In Vitro Experiment on Percutaneous Absorption of Perfluorooctanoate (PFOA) and Perfluorooctane Sulfonate (PFOS)

  • 摘要: 目的 探讨全氟辛酸(PFOA)和全氟辛烷磺酸(PFOS)是否能体外经皮吸收及其吸收特征。

    方法 采用Franz扩散装置, 以 10 μmol/L的 PFOA和 PFOS的混合溶液(1:1)作为供体液, 用 HPLC-MS测定两种化合物的单位面积SD大鼠离体皮肤的累积渗透量, 分别计算其稳态渗透速率、渗透滞后时间和渗透系数。

    结果 各透过时间点受体液中均可检测到 PFOA 和 PFOS; PFOA 和 PFOS的 12 h累积渗透量分别为(4.97& #177;1.80)、(1.76& #177;1.06)μg/cm2, 稳态渗透速率分别为(0.48& #177;0.19)、(0.16& #177;0.06)μg/cm2& #183;h, 渗透滞后时间分别为(0.31& #177;0.18)、(0.50& #177;0.06)h, 渗透系数分别为(11.48& #177;4.51)& #215;10-2、(3.17& #177;1.22)& #215;10-2 cm/h。

    结论 PFOA和 PFOS均能够经皮吸收, 前者稳态渗透快于后者, 渗透系数大于后者, 渗透滞后时间明显小于后者。该研究确定了 PFOA和 PFOS的体外经皮吸收特征, 为暴露量评定和危险性评价提供了有意义的基础研究数据。

     

    Abstract: Objective To study the percutaneous penetration of perfluorooctanoate acid(PFOA)and perfluorooctane sulfonate(PFOS)as well as its absorption characteristics through excised rat skin in vitro.

    Methods Using finite dose of 10μmol/L PFOA and PFOS mixed solution as body fluids combined with Franz diffusion cells and HPLC-MS method, the differences of accumulative permeating amounts between PFOA and PFOS were measured, and their steady-state penetration rates, lag time and permeability coefficients were calculated.

    Results PFOA and PFOS could be detected in receptor fluids at each time point, the calculated PFOA and PFOS cumulative permeation amounts at 12 h, steady-state penetration rates, lag time, permeability coefficients were(4.97& #177;1.80)and(1.76& #177;1.06)μg/cm2(0.48& #177;0.19)and(0.16& #177;0.06)μg/cm2& #183;h,(0.31& #177;0.18)and(0.50& #177;0.06)h(, 11.48& #177;4.51)& #215;10-2 and(3.17& #177;1.22)& #215;10-2 cm/h respectively.

    Conclusion PFOA and PFOS were able to conduct percutaneous absorption, the formers penetrated at a faster and higher rate with significant shorter lag time. The study identified the characteristics of PFOA and PFOS percutaneous absorption, provided basic research data for exposure and risk assessment.

     

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