杨文海, 杨瑾, 王发选, 王亚娜, 汪岭, 陈莉莉, 宋琦如. 转化生长因子-β1基因509C/T多态性在宁夏煤工尘肺人群中的分布[J]. 环境与职业医学, 2010, 27(11): 670-672.
引用本文: 杨文海, 杨瑾, 王发选, 王亚娜, 汪岭, 陈莉莉, 宋琦如. 转化生长因子-β1基因509C/T多态性在宁夏煤工尘肺人群中的分布[J]. 环境与职业医学, 2010, 27(11): 670-672.
YANG Wen-hai , YANG Jin , WANG Fa-xuan , WANG Ya-na , WANG Ling , CHEN Lili , SONG Qi-ru . The Distribution of TGF-β1 Gene-509 C/T Polymorphism in Coal Worker's Pneumoconiosis Patients in Ningxia Area[J]. Journal of Environmental and Occupational Medicine, 2010, 27(11): 670-672.
Citation: YANG Wen-hai , YANG Jin , WANG Fa-xuan , WANG Ya-na , WANG Ling , CHEN Lili , SONG Qi-ru . The Distribution of TGF-β1 Gene-509 C/T Polymorphism in Coal Worker's Pneumoconiosis Patients in Ningxia Area[J]. Journal of Environmental and Occupational Medicine, 2010, 27(11): 670-672.

转化生长因子-β1基因509C/T多态性在宁夏煤工尘肺人群中的分布

The Distribution of TGF-β1 Gene-509 C/T Polymorphism in Coal Worker's Pneumoconiosis Patients in Ningxia Area

  • 摘要: 目的 探讨转化生长因子-β1(TGF-β1)基因509 C/T多态性在宁夏汉族煤工尘肺(coal worker's pneumoconiosis,CWP)人群中的分布情况。

    方法 从宁夏某煤业集团所属煤矿已确诊的病例中随机抽取110名煤工尘肺病例(煤工尘肺组)、110名煤尘接触者(煤尘接触组)和110名当地不接尘的正常居民(非煤尘接触组)为研究对象,采集静脉血,使用聚合酶链反应-限制片段长度多态性(PCR-RFLP)分析TGF-β1基因509 CT位点多态性。

    结果 煤工尘肺组、煤尘接触组及非煤尘接触组平均年龄分别为(48.30& #177;13.16)岁、(43.86& #177;4.20)岁、(41.73& #177;5.45)岁。煤工尘肺组和煤尘接触组工龄分别为(17.40& #177;7.84)年和(16.95& #177;6.10)年。工种构成:主掘进工在煤工尘肺组和煤尘接触组中分别占48.2%和49.1%。煤工尘肺组和煤尘接触组及非煤尘接触组中吸烟烟龄分别为(10.48& #177;10.12)年、(11.34& #177;10.48)年、(12.65& #177;11.53)年;接尘工龄、工种构成、吸烟烟龄均无差别,均P > 0.05。TGF-β1基因509 C/T位点多态性在煤工尘肺组、煤尘接触组及非煤尘接触组中所占比例分别为51.8%、30.9%、25.5%;等位基因频率T在煤工尘肺组、煤尘接触组及非煤尘接触组中所占比例分别为40.90%、23.20%、19.10%;煤工尘肺组与煤尘接触组比较,TGF-β1基因中CT基因型差异有统计学意义(P<0.01)。煤工尘肺组不同期别人群的等位基因频率差异有统计学意义(P<0.01)。

    结论 TGF-β1-509 C/T位点多态性在宁夏地区煤工尘肺者中的分布可能不同。

     

    Abstract: Objective To investigate gene polymorphisms of transforming growth factor-beta1(TGF-β1)-509 C/T in patients with coal worker's pneumoconiosis (CWP).

    Methods Total of 110 patients with documented CWP and 110 workers exposed to dust and 110 local residents without dust exposure as control group were recruited. The TGF-β1-509 C/T genotypes were determined by PCR-restriction fragment length polymorphism of vein blood samples.

    Results The average ages of CWP group, the dust exposure group and the control group were (48.30& #177;13.16) (Mean& #177;SD),(43.86& #177;4.20), and (41.73& #177;5.45)years respectively; The average working ages of the CWP group and the coal dust exposure group were (17.40& #177;7.84)years (Mean& #177;SD)and (16.95& #177;6.10) years respectively. 48.2% of the CWP group and 49.1% of the dust exposure group were drifters. The durations of smoking varied in the CWP group, the dust exposure group and the control group(10.48& #177;10.12)years (Mean& #177;SD),(11.34& #177;10.48)years,(12.65& #177; 11.53)years, respectively.The length of dust expsure and job title distribution were found no statistically difference between worker's groups. Smoking ages were not difference in all three groups. The percentage of TGF-β1-509 C/T genotype polymorphism were 51.8%、30.9% and 25.5% for the CWP group, the dust exposure group and the control group respectively.The allele frequency of T in the CWP group, the dust exposure group and the control group were 40.9%、23.2%、19.1% respectively. Both TGF-β1-509 C/T genotypes and alleles frequencies were significantly higher in the CWP patients than in the controls (P < 0.05).

    Conclusion TGF-β1-509 C/T polymorphisms distributions were different in workers with CWP and residents without dust exposure.

     

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