氯化钕对小鼠骨髓细胞的遗传毒作用
Genetic Toxicity of Neodymium Chloride on Marrow Cell of Mice
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摘要:
目的 探讨氯化钕对小鼠骨髓细胞遗传物质的影响。 方法 小鼠腹腔注射氯化钕染毒,采用微核测试技术和单细胞凝胶电泳技术检测小鼠骨髓红细胞微核率和彗星细胞DNA拖尾率。微核试验设置20、40、80、160 mg/kg 4个剂量组,彗星试验设置20、40、80 mg/kg 3个剂量组。 结果 在20~160 mg/kg剂量范围内,随着氯化钕剂量的增加小鼠骨髓细胞微核率(FMN)升高(rFMN=0.956);在20~80 mg/kg剂量范围内,彗星细胞DNA拖尾率(PTC)和尾长(TL)随着剂量的增加而增加,存在剂量-效应关系(rPTC=0.946,rTL=0.997)。 结论 氯化钕对小鼠骨髓细胞具有一定的遗传毒作用。 Abstract:Objective The toxic effect of neodymium chloride on genetic material of mouse bone marrow cells was explored. Methods Neodymium chloride was induced to mice via intraperitoneal injection, dose groups were 20, 40, 80, 160 mg/kg for micronucleus test and 20, 40, 80 mg/kg for comet assay. Regular micronucleus test technology and single-cell gel electrophoresis micronucleated erythrocytes were applied on mouse bone marrow samples. Micronucleus and comet tail of DNA cells were calculated. Results In micronucleus test, micronucleus rates of mouse bone marrow were highly correlated with the doses of neodymium chloride (rFMN=0.956); the cell DNA comet tail ratio and tail length showed dose-response relationships with the doses of neodymium chloride (rPTC=0.946, rTL=0.997, respectively). Conclusion The study suggested that neodymium chloride may have a genetic toxic effect on marrow cell of mice.
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