张红丹, 徐诚, 王彤, 张娟, 宋宁慧. 南京两医院体检人群尿液中有机磷酸酯代谢物与氧化应激水平的关联[J]. 环境与职业医学, 2021, 38(10): 1106-1112. DOI: 10.13213/j.cnki.jeom.2021.21239
引用本文: 张红丹, 徐诚, 王彤, 张娟, 宋宁慧. 南京两医院体检人群尿液中有机磷酸酯代谢物与氧化应激水平的关联[J]. 环境与职业医学, 2021, 38(10): 1106-1112. DOI: 10.13213/j.cnki.jeom.2021.21239
ZHANG Hongdan, XU Cheng, WANG Tong, ZHANG Juan, SONG Ninghui. Associations of urinary organophosphate metabolites with oxidative stress levels in physical examination population in two hospitals of Nanjing[J]. Journal of Environmental and Occupational Medicine, 2021, 38(10): 1106-1112. DOI: 10.13213/j.cnki.jeom.2021.21239
Citation: ZHANG Hongdan, XU Cheng, WANG Tong, ZHANG Juan, SONG Ninghui. Associations of urinary organophosphate metabolites with oxidative stress levels in physical examination population in two hospitals of Nanjing[J]. Journal of Environmental and Occupational Medicine, 2021, 38(10): 1106-1112. DOI: 10.13213/j.cnki.jeom.2021.21239

南京两医院体检人群尿液中有机磷酸酯代谢物与氧化应激水平的关联

Associations of urinary organophosphate metabolites with oxidative stress levels in physical examination population in two hospitals of Nanjing

  • 摘要: 背景

    有机磷酸酯(OPEs)被广泛用作阻燃剂和添加剂,已成为环境介质中普遍存在的污染物。尿液中磷酸二酯已被确定为评估人类OPEs暴露的生物标志物。

    目的

    检测人群尿液中7种OPEs二酯代谢物和人体氧化应激生物标志物8- 羟基-2’- 脱氧鸟苷(8-OHdG)的水平,分析二者之间的关联。

    方法

    调查南京两医院参加健康体检的304名普通成人,女性135名和男性169名,年龄为18~65岁。通过高效液相色谱串联质谱法测定尿液中磷酸二乙酯(DEP)、磷酸二正丁酯(DnBP)、磷酸二苯酯(DPhP)、双(2- 氯乙基)磷酸酯(BCEP)、双(1- 氯-2- 丙基)磷酸酯(BCPP)、双(丁氧乙基)磷酸酯(BBEP)、双(1,3- 二氯-2- 丙基)磷酸酯(BDCPP)和8-OHdG的含量。通过Pearson相关性检验和多元线性回归分析检验尿液OPEs代谢产物和8-OHdG之间的相关性。

    结果

    BCPP几何均数(GM):4.88 μg·g-1和DnBP(GM:4.61 μg·g-1)是尿液最丰富的OPEs代谢产物。尿液DEP、DnBP、BCPP、BBEP和BDCPP水平存在性别差异(P < 0.05):男性尿液DnBP、BCPP和BDCPP高于女性(GM:5.83、6.44、0.86 μg·g-1 vs 5.47、3.42、0.69 μg·g-1),女性尿液DEP和BBEP高于男性(GM:7.44、5.25 μg·g-1 vs 0.67、0.80 μg·g-1)。Pearson相关性检验显示尿液8-OHdG与DEP(r=0.4170.219)、DnBP(r=0.417)、DPhP(r=0.4170.308)、BCEP(r=0.4170.420)、BDCPP(r=0.4170.287)和Σ7OPEMs(r=0.4170.276)均呈正相关(均P < 0.01);性别分层后,男性尿液8-OHdG与DEP(r=0.4170.240)、DnBP(r=0.4170.436)、DPhP(r=0.4170.367)、BCEP(r=0.4170.448)、BDCPP(r=0.4170.348)和Σ7OPEMs(r=0.4170.327)均呈正相关(均P < 0.05),女性尿液8-OHdG与BBEP呈正相关(r=0.4170.233,P < 0.05)。进一步校正性别和年龄后,多元线性回归分析显示尿液8-OHdG与DEP(b=0.182)、DnBP(b=0.418)、DPhP(b=0.303)、BCEP(b=0.418)、BDCPP(b=0.284)和Σ7OPEMs(b=0.281)均呈正向关联(均P < 0.05)。

    结论

    本研究发现DEP、DnBP、DPhP、BCEP和BDCPP与8-OHdG水平升高相关,表明成年人暴露于OPEs可能会在代谢过程中引起氧化应激。

     

    Abstract: Background

    Organophosphate esters (OPEs) are widely used as flame retardants and additives, and have become a ubiquitous pollutant in various environmental media. Phosphodiesters in urine have been identified as a biomarker for assessing human OPEs exposure.

    Objective

    This study is conducted to detect the levels of seven human urinary OPEs diester metabolites and oxidative stress biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG), and assess their relationships.

    Methods

    The cross-sectional study included a total of 304 adults, 135 women and 169 men, aged 18-65 years, who participated in health checkups in two hospitals in Nanjing. The levels of urinary diethyl phosphate (DEP), di-n-butyl phosphate (DnBP), diphenyl phosphate (DPhP), bis(2-chloroethyl) phosphate (BCEP), bis(1-chloro-2-propyl) phosphate (BCPP), bis(2-butoxyethyl) phosphate (BBEP), bis(1, 3-dichloro-2-propyl) phosphate (BDCPP), and 8-OHdG were measured by high-performance liquid chromatography coupled with tandem mass spectrometry. Pearson correlation test and multiple linear regression analysis were used to test the correlation between urinary OPEs metabolites and 8-OHdG.

    Results

    BCPP geometric mean (GM): 4.88 μg·g-1 and DnBP (GM: 4.61 μg·g-1) were the most abundant urinary OPEs metabolites. There were gender differences in urine DEP, DnBP, BCPP, BBEP, and BDCPP levels (P < 0.05): The DnBP, BCPP, and BDCPP levels in urine were higher in males (GM: 5.83, 6.44, 0.86 μg·g-1 vs 5.47, 3.42, 0.69 μg·g-1), while the DEP and BBEP levels in urine were higher in females (GM: 7.44, 5.25 μg·g-1 vs 0.67, 0.80 μg·g-1). The pearson correlation test results showed that urinary 8-OHdG was positively associated with DEP (r=0.4170.219), DnBP (r=0.4170.417), DPhP (r=0.4170.308), BCEP (r=0.4170.420), BDCPP (r=0.4170.287), and Σ7OPEMs (r=0.4170.276) (all P < 0.01). After gender stratification, male urinary 8-OHdG was positively correlated with DEP (r=0.4170.240), DnBP (r=0.4170.436), DPhP (r=0.4170.367), BCEP (r=0.4170.448), BDCPP (r=0.4170.348), and Σ7OPEMs (r=0.4170.327) (all P < 0.05), while female urinary 8-OHdG and BBEP were positively correlated (r=0.4170.233, P < 0.05). After further adjusting for gender and age, the results of multiple linear regression analysis showed that urinary 8-OHdG was positively correlated with DEP (b=0.182), DnBP (b=0.418), DPhP (b=0.303), BCEP (b=0.418), BDCPP (b=0.284), and Σ7OPEMs (b=0.281) (all P < 0.05).

    Conclusion

    The study findings show that DEP, DnBP, DPhP, BCEP, and BDCPP are associated with increased levels of 8-OHdG, indicating that being exposed to OPEs may cause oxidative stress during their metabolism in adults.

     

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