陈章健, 韩硕, 郑湃, 周淑佩, 贾光. 纳米二氧化钛与葡萄糖亚慢性经口联合暴露对幼年大鼠凝血功能的影响[J]. 环境与职业医学, 2020, 37(5): 503-507, 519. DOI: 10.13213/j.cnki.jeom.2020.19862
引用本文: 陈章健, 韩硕, 郑湃, 周淑佩, 贾光. 纳米二氧化钛与葡萄糖亚慢性经口联合暴露对幼年大鼠凝血功能的影响[J]. 环境与职业医学, 2020, 37(5): 503-507, 519. DOI: 10.13213/j.cnki.jeom.2020.19862
CHEN Zhang-jian, HAN Shuo, ZHENG Pai, ZHOU Shu-pei, JIA Guang. Effect of subchronic combined oral exposure to titanium dioxide nanoparticles and glucose on blood coagulation in young rats[J]. Journal of Environmental and Occupational Medicine, 2020, 37(5): 503-507, 519. DOI: 10.13213/j.cnki.jeom.2020.19862
Citation: CHEN Zhang-jian, HAN Shuo, ZHENG Pai, ZHOU Shu-pei, JIA Guang. Effect of subchronic combined oral exposure to titanium dioxide nanoparticles and glucose on blood coagulation in young rats[J]. Journal of Environmental and Occupational Medicine, 2020, 37(5): 503-507, 519. DOI: 10.13213/j.cnki.jeom.2020.19862

纳米二氧化钛与葡萄糖亚慢性经口联合暴露对幼年大鼠凝血功能的影响

Effect of subchronic combined oral exposure to titanium dioxide nanoparticles and glucose on blood coagulation in young rats

  • 摘要: 背景

    动物实验结果表明纳米二氧化钛(TiO2)经口暴露可以影响机体血液系统的凝血功能。TiO2作为食品添加剂,常用于高糖食物,且由于儿童喜食甜食而成为其高暴露人群。

    目的

    探讨纳米TiO2与葡萄糖联合暴露对幼年大鼠凝血功能的影响。

    方法

    3周龄清洁级SD大鼠80只,适应性喂养一周后,随机分为8组(每组10只,雌雄各半)。采用4×2析因设计方法,大鼠每天灌胃给予0、2、10、50 mg·kg-1(以体重计)纳米TiO2,分别加或不加1.8 g·kg-1(以体重计)葡萄糖。实验期间,大鼠体重每周监测一次。染毒90 d后进行凝血指标检测,包括活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)和纤维蛋白原(FIB)。利用响应面模型和一般线性模型进行交互作用分析。

    结果

    纳米TiO2与葡萄糖经口暴露对大鼠体重未产生影响。纳米TiO2单独暴露时,仅雌性大鼠在10mg·kg-1剂量下APTT(33.90±2.05)s高于对照组(25.88±3.59)s(P < 0.05)。纳米TiO2与葡萄糖联合暴露时,雌性大鼠在50mg·kg-1剂量下,与对照组相比APTT(44.10±18.12)s vs(25.88±3.59)s和PT(26.40±4.21)s vs(21.38±1.04)s增加;雄性大鼠在2mg·kg-1和10mg·kg-1剂量下,与对照组相比,PT(22.64±1.53)s和(21.68±2.19)s vs(24.96±1.05)s降低,并在50mg·kg-1剂量下,与对照组相比,FIB(1.47±0.07)g·L-1 vs(1.76±0.08)g·L-1降低(P < 0.05)。交互作用分析发现,在雄性大鼠中,纳米TiO2和葡萄糖在影响APTT和FIB时存在拮抗作用(P < 0.05)。

    结论

    亚慢性纳米TiO2和葡萄糖联合经口暴露可以对幼年SD大鼠凝血功能产生影响,两者存在拮抗作用。纳米TiO2和葡萄糖经口联合暴露比纳米TiO2单独暴露效应更加明显。实际暴露情况下,纳米TiO2与糖的联合暴露效应需引起关注。

     

    Abstract: Background

    Animal experiments have shown that oral exposure to titanium dioxide (TiO2) nanoparticles can affect hematologic system. TiO2 nanoparticles are often found in high sugar food as food additives, and children are highly exposed to them because they have a sweet tooth.

    Objective

    This study aims to explore the effect of combined oral exposure to TiO2 nanoparticles and glucose on blood coagulation in young rats.

    Methods

    Eighty three-week-old clean SD rats were randomly divided into eight groups (10 rats in each group, half male and half female) after one week of adaptive feeding. By a 4×2 factorial design, the rats were exposed to TiO2 nanoparticles through intragastric administration at 0, 2, 10, and 50 mg·kg-1 (body weight) with or without 1.8 g·kg-1 (body weight) glucose daily for 90 days. During the experiment, the body weight of the rats was monitored once a week. After 90 days of exposure, blood coagulation indices such as activated partial thromboplastin time (APTT), prothrombin time (PT), and fibrinogen (FIB) were detected. Response surface model and general linear model were used for interaction analysis.

    Results

    Exposure to TiO2 nanoparticles and glucose did not change body weight of the rats. Only APTT in the female rats exposed to 10 mg·kg-1 TiO2 nanoparticles(33.90±2.05) s was higher than that in the control group(25.88±3.59) s (P < 0.05). The APTT(44.10±18.12) s vs (25.88±3.59) s and PT(26.40±4.21) s vs (21.38±1.04) s were increased in the female rats exposed to 50mg·kg-1 TiO2 nanoparticles and glucose compared with the control group, and PT(22.64±1.53) s and (21.68±2.19)s vs (24.96±1.05)s was decreased in the male rats exposed to 2mg·kg-1 and 10mg·kg-1 TiO2 nanoparticles and glucose and FIB(1.47±0.07)g·L-1 vs (1.76±0.08)g·L-1 was decreased in the male rats exposed to 50 mg·kg-1 TiO2 nanoparticles and glucose compared with the control group (P < 0.05). The interaction analysis results showed that TiO2 nanoparticles and glucose had a significant antagonistic effect on APTT and FIB in male rats (P < 0.05).

    Conclusion

    Subchronic combined oral exposure to TiO2 nanoparticles and glucose have an obvious antagonistic effect on blood coagulation in young SD rats. The combined effect of oral exposure to TiO2 nanoparticles and glucose is more obvious than that of TiO2 nanoparticles alone. In the case of real consumer exposure, the combined exposure effect of TiO2 nanoparticles and sugar needs concern.

     

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