陈益钦, 管芳圆, 申琪, 郭雨柔, 席煜, 王文祥. 大豆异黄酮对成年期大鼠卵巢卵泡发育的影响[J]. 环境与职业医学, 2019, 36(9): 813-817. DOI: 10.13213/j.cnki.jeom.2019.19220
引用本文: 陈益钦, 管芳圆, 申琪, 郭雨柔, 席煜, 王文祥. 大豆异黄酮对成年期大鼠卵巢卵泡发育的影响[J]. 环境与职业医学, 2019, 36(9): 813-817. DOI: 10.13213/j.cnki.jeom.2019.19220
CHEN Yi-qin, GUAN Fang-yuan, SHEN Qi, GUO Yu-rou, XI Yi, WANG Wen-xiang. Effects of soy isoflavone on ovarian follicle development in adult rats[J]. Journal of Environmental and Occupational Medicine, 2019, 36(9): 813-817. DOI: 10.13213/j.cnki.jeom.2019.19220
Citation: CHEN Yi-qin, GUAN Fang-yuan, SHEN Qi, GUO Yu-rou, XI Yi, WANG Wen-xiang. Effects of soy isoflavone on ovarian follicle development in adult rats[J]. Journal of Environmental and Occupational Medicine, 2019, 36(9): 813-817. DOI: 10.13213/j.cnki.jeom.2019.19220

大豆异黄酮对成年期大鼠卵巢卵泡发育的影响

Effects of soy isoflavone on ovarian follicle development in adult rats

  • 摘要: 背景 大豆异黄酮具有类似内源性雌激素的结构和功能,在豆类制品中广泛存在。既往研究表明不同发育时期大豆异黄酮暴露对女性生殖系统具有不同的影响,但关于成年期大豆异黄酮暴露的研究尚未见相关报道。

    目的 探讨成年期大豆异黄酮暴露对大鼠卵巢卵泡发育的影响,为成年期大豆异黄酮的合理利用提供科学依据。

    方法 44只3月龄清洁级雌性Wistar大鼠,按体重随机分为对照组和低、中、高大豆异黄酮剂量组(25、50、100 mg/kg),每组11只,每日灌胃1次,持续6个月。干预结束后取卵巢称重并计算脏器系数;观察卵巢组织学变化并计算卵泡构成比;测定血清抗苗勒氏管激素(AMH)水平;测定卵巢AMH、雌激素受体β(ERβ)、卵特异性连接组蛋白(H1foo)、干细胞因子(SCF)及其受体(c-Kit)的mRNA表达水平。

    结果 与对照组相比,各剂量组体重、卵巢重量及其脏器系数差异均无统计学意义(P>0.05)。与对照组相比:低剂量组窦前卵泡构成比升高(12.2% vs 8.6%);中剂量组有腔卵泡构成比升高(40.7% vs 30.3%),但黄体构成比降低(7.4% vs 19.1%);高剂量组窦前卵泡构成比降低(3.4% vs 8.6%),但闭锁卵泡构成比升高(53.8% vs 42.0%);差异均具有统计学意义(P < 0.05)。各剂量组血清AMH水平差异无统计学意义(P>0.05);但低、中剂量组AMH mRNA表达水平下降,高剂量组表达水平上调(对照组和低、中、高剂量组分别为1.00±0.03、0.58±0.03、0.53±0.03、2.82±0.37,P < 0.05)。与对照组(0.92±0.07)相比,低、中、高剂量组c-Kit mRNA表达水平均上调(分别为1.19±0.01、1.50±0.07、1.80±0.15,P < 0.05)。同样,低剂量组SCF mRNA及高剂量组H1fooERβ mRNA表达水平相较对照组也上调分别为(2.08±0.06)vs(1.19±0.29),(1.46±0.03)vs(1.03±0.13),(1.29±0.26)vs(0.92±0.09),P < 0.05。

    结论 成年期大鼠25 mg/kg及以上的大豆异黄酮暴露可通过影响卵巢细胞因子的表达进而影响卵巢卵泡发育。

     

    Abstract: Background Soy isoflavone has similar structure and function to endogenous estrogen and is widely found in soy products. Previous studies have shown that soy isoflavone exposure at different developmental stages has different effects on female reproductve system, but there are no relevant reports on adult soy isoflavone exposure.

    Objectve This animal experiment explores the effects of adulthood soy isoflavone exposure on ovarian follicle development in rats, aiming to provide a scientfc basis for the ratonal use of soy isoflavone in adulthood.

    Methods Forty-four three-month-old clean female Wistar rats were randomly divided into control group, and low-, medium-, and high-dose soy isoflavone groups (25, 50, and 100 mg/kg), 11 rats per group, and received intragastric administraton once a day for six months. Afer that, the ovary was weighed and organ coefcient was calculated; the histological changes of ovary were observed and the follicle proporton was calculated; the ant-Muller hormone (AMH) level in serum was determined; the mRNA expression levels of AMH, estrogen receptor β (ERβ), oocyte-specifc linker histone (H1foo), stem cell factor (SCF), and its receptor (c-Kit) in ovary were determined.

    Results Compared with the control group, there was no signifcant difference in body weight, ovary weight, and its organ coefcient in the three dose groups (P>0.05). Compared with the control group, the proporton of pre-antral follicles in the low-dose group was increased (12.2% vs 8.6%); the proporton of antral follicles was increased (40.7% vs 30.3%), but the proporton of corpus luteum was decreased (7.4% vs 19.1%) in the middle-dose group; the proportion of pre-antral follicles was decreased (3.4% vs 8.6%), while the proporton of atresia follicles was increased (53.8% vs 42.0%) in the high-dose group; all the differences were statstcally signifcant (P < 0.05). There was no signifcant difference in serum AMH levels (P>0.05) across all groups, but the expression levels of AMH mRNA were decreased in the low-and medium-dose groups and increased in the high-dose group (control:1.00±0.03; low-dose:0.58±0.03; mediumdose:0.53±0.03; high-dose:2.82±0.37; P < 0.05). Compared with the control group (0.92±0.07), the expression levels of c-Kit mRNA were up-regulated in the low-, medium-, and high-dose groups (1.19±0.01, 1.50±0.07, and 1.80±0.15, respectvely, P < 0.05), and the level of SCF mRNA in the low-dose group and the levels of H1foo and ERβ mRNA in the high-dose group were also up-regulated(2.08±0.06) vs (1.19±0.29), (1.46±0.03) vs (1.03±0.13), and (1.29±0.26) vs (0.92±0.09), respectvely, P < 0.05.

    Conclusion Exposure to soy isoflavone at 25 mg/kg and above in adult rats could affect ovarian follicle development through affectng the expression of ovarian cytokines.

     

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