张春妹, 杨永寿, 肖培云. 美洲大蠊活性组分对矽尘致大鼠肺纤维化的影响[J]. 环境与职业医学, 2018, 35(12): 1134-1138. DOI: 10.13213/j.cnki.jeom.2018.18156
引用本文: 张春妹, 杨永寿, 肖培云. 美洲大蠊活性组分对矽尘致大鼠肺纤维化的影响[J]. 环境与职业医学, 2018, 35(12): 1134-1138. DOI: 10.13213/j.cnki.jeom.2018.18156
ZHANG Chun-mei, YANG Yong-shou, XIAO Pei-yun. Influence of active ingredients extracted from Periplaneta Americana on pulmonary fibrosis in silica-exposed rats[J]. Journal of Environmental and Occupational Medicine, 2018, 35(12): 1134-1138. DOI: 10.13213/j.cnki.jeom.2018.18156
Citation: ZHANG Chun-mei, YANG Yong-shou, XIAO Pei-yun. Influence of active ingredients extracted from Periplaneta Americana on pulmonary fibrosis in silica-exposed rats[J]. Journal of Environmental and Occupational Medicine, 2018, 35(12): 1134-1138. DOI: 10.13213/j.cnki.jeom.2018.18156

美洲大蠊活性组分对矽尘致大鼠肺纤维化的影响

Influence of active ingredients extracted from Periplaneta Americana on pulmonary fibrosis in silica-exposed rats

  • 摘要: 目的 探讨美洲大蠊抗肺纤维化活性组分(ML-HB)对矽肺大鼠纤维化的影响。

    方法 成年雄性SD大鼠随机分为正常组、模型组、ML-HB高、中、低剂量组,每组32只。除正常组外,其余各组采用气管暴露法注入SiO2混悬液复制矽肺模型,模型复制后第3天,经腹腔给药干预,ML-HB高、中、低剂量组以120、60、30 mg(/kg·d)给药,模型组给予等剂量生理盐水,正常组无特殊处理。给药后第30、45、60、75天分别处死8只大鼠,并观察大鼠肺组织外观,采用免疫组织化法观察α-平滑肌肌动蛋白(α-SMA)与I型胶原(COL-I)的表达,ELISA法检测肺匀浆中肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)的质量体积浓度。

    结果 模型组大鼠肺组织体积明显增大,颜色暗红且不均匀,表面被灰白色斑状结节覆盖,触之有沙粒感,无弹性,表明矽肺模型复制成功。与正常组比,各时间点模型组α-SMA、COL-I阳性表达增强,TNF-α和TGF-β1质量体积浓度升高(P<0.05)。与模型组相比,ML-HB各剂量组α-SMA、COL-I表达有所降低,中剂量组改善最明显;ML-HB中剂量组TNF-α、TGF-β1质量体积浓度均降低(P<0.05)。

    结论 ML-HB可降低SiO2染毒后肺组织中α-SMA、COL-I、TNF-α和TGF-β1的表达,延缓肺组织炎性病变、干预胶原蛋白的沉积,从而抑制肺纤维化的进一步发展。

     

    Abstract: Objective To study the influence of active ingredients (ML-HB) of anti-pulmonary fibrosis extracted from Periplaneta Americana on pulmonary fibrosis in rats.

    Methods Adult male SD rats were randomly divided into normal group, model group, and ML-HB high-, medium-, and low-dose groups, with thirty-two rats in each group. Except for the normal group, all groups were treated with SiO2 suspension by intratracheal administration; then the rats were intervened with ML-HB at 120, 60, and 30 mg/(kg·d) by intraperitoneal administration on the third day after the SiO2 treatment, the model group was given isodose saline, and the normal group was given no additional treatment. Eight rats in each group were sacrificed on the 30th, 45th, 60th, and 75th days respectively after the intervention, rat lung tissues were observed, the expressions of alpha-smooth muscle actin (α-SMA) and collagen type I (COL-I) were observed by immunohistochemistry, and the concentrations of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in lung homogenate were detected by ELISA.

    Results The model group showed increased lung volume, dark red and uneven lung tissues, gray-white plaque nodules on the surface of the sampled lung tissues, and a sense of sandiness and inelasticity, indicating that the silicosis model was established successfully. The expressions of α-SMA and COL-I and the concentrations of TNF-α and TGF-β1 in the lung tissues of the model group were increased (P < 0.05) at different time points compared with the normal group. The expressions of α-SMA and COL-I in the ML-HB groups were all lower than those in the model group, especially the ML-HB medium-dose group (P < 0.05). The concentrations of TNF-α and TGF-β1 in the ML-HB medium-dose groups were significantly lower than those in the model group (P < 0.05).

    Conclusion ML-HB can decrease the expressions of α-SMA, COL-I, TNF-α, and TGF-β1 in lung tissues post SiO2 exposure, delay the inflammatory lesions of lung tissues, and interfere with the deposition of collagen, thus intervene in further development of pulmonary fibrosis.

     

/

返回文章
返回