朱航榉, 王锋, 杨贤, 王兆丹, 夏慧, 成晨, 孙桂菊. 不同油脂对小鼠肠道菌群的影响[J]. 环境与职业医学, 2017, 34(11): 995-998. DOI: 10.13213/j.cnki.jeom.2017.17294
引用本文: 朱航榉, 王锋, 杨贤, 王兆丹, 夏慧, 成晨, 孙桂菊. 不同油脂对小鼠肠道菌群的影响[J]. 环境与职业医学, 2017, 34(11): 995-998. DOI: 10.13213/j.cnki.jeom.2017.17294
ZHU Hang-ju, WANG Feng, YANG Xian, WANG Zhao-dan, XIA Hui, CHENG Chen, SUN Gui-ju. Effects of different oils on intestinal microbiota of mice[J]. Journal of Environmental and Occupational Medicine, 2017, 34(11): 995-998. DOI: 10.13213/j.cnki.jeom.2017.17294
Citation: ZHU Hang-ju, WANG Feng, YANG Xian, WANG Zhao-dan, XIA Hui, CHENG Chen, SUN Gui-ju. Effects of different oils on intestinal microbiota of mice[J]. Journal of Environmental and Occupational Medicine, 2017, 34(11): 995-998. DOI: 10.13213/j.cnki.jeom.2017.17294

不同油脂对小鼠肠道菌群的影响

Effects of different oils on intestinal microbiota of mice

  • 摘要: 目的 研究不同油脂对肠道菌群的影响。

    方法 采用C57BL/6雄性小鼠(6周龄,体重16~18 g),每组12只,分为7组,对照组采用脂肪能量占总能量10%的普通饲料饲养,其余6组用D12451高脂饲料进行饲养,各组高脂饲料中的油脂分别为猪油、大豆油、24度棕榈油、33度棕榈油、混合油(含大豆油与棕榈油,质量比=0.9:1)、橄榄油,连续干预12周后眼框采血,检测总胆固醇和三酰甘油等血脂指标,处死后采集结肠内的残留排泄物,采用16S rRNA扩增子测序技术进行测序,进行操作分类单元聚类和物种分类分析。采用MetaStat方法分析物种丰度数据,得到P值,校正后得到q值,筛选具有差异的物种。

    结果 实验组总胆固醇和三酰甘油高于对照组。混合油组放线菌门物种相对丰度高于对照组(q<0.05)。猪油组、33度棕榈油组疣微菌门物种相对丰度高于对照组(q<0.05)。

    结论 长期的高脂膳食会对小鼠肠道菌群产生影响。大豆油、橄榄油、24度棕榈油不会引起肠道菌群的异常。

     

    Abstract: Objective To study the effects of different oils on intestinal microbiota.

    Methods C57BL/6 male mice (6 weeks old, 16-18 g) were divided into 7 groups with 12 mice in each group. The control group was fed with an ordinary diet with a 10% fat-energy ratio, and the other groups were fed with a high-fat diet (D12451) added with lard, soybean oil, 24 degrees palm oil, 33 degrees palm oil, mixture oil (soybean oil and palm oil with a mass ratio of 0.9:1), and olive oil, respectively. After a 12-week diet intervention, blood samples were collected from the orbit of mice to detect total cholesterol and triglyceride. Residual waste was removed from colon to conduct 16S rRNA amplicon sequencing for cluster analysis and speciation analysis. MetaStat was used to analyze species abundance, obtain P value and q value (adjusted P value), and screen species with significant differences.

    Results The total cholesterol and triglyceride of the experimental groups were higher than those of the control group. The mixed oil group showed a higher level of relative abundance of Actinobacteria than the control group (q < 0.05). The lard group and the 33 degrees palm oil group showed higher levels of relative abundance of Verrucomicrobia than the control group (q < 0.05).

    Conclusion A long-term high-fat diet could affect the intestinal flora of mice. Soybean oil, olive oil, and 24 degrees palm oil do not cause abnormalities in intestinal microbiota.

     

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