矽尘对小鼠肺组织TLR4 mRNA与RelA mRNA表达的影响
Effects of silica dust on expression of TLR4 mRNA and RelA mRNA in lung tissue in mice
-
摘要:
目的 探讨矽尘对小鼠肺组织TOLL样受体4（TLR4）mRNA与核因子-κB p65（RelA）mRNA表达的影响。
方法 将40只小鼠随机分成4组，每组10只，分别为2、4、8 h暴露组（即每天吸入染尘2、4、8 h）和对照组（不染尘）。染尘20 d后，取肺组织，应用实时荧光定量PCR法（RT-qPCR）测定各组TLR4 mRNA和RelA mRNA的表达。
结果 对照组与3个暴露组TLR4 mRNA表达量的中位数分别为0.89、1.22、4.89、7.21，4个组间的差异有统计学意义（χ2=4.93，P=0.02）；4、8 h暴露组与对照组的差异有统计学意义（P < 0.05），2 h暴露组与对照组的差异无统计学意义（P>0.05）。对照组和3个暴露组RelA mRNA表达量分别为0.96、1.33、2.23、3.04，4个组间差异有统计学意义（χ2=6.35，P=0.00）；4、8 h暴露组与对照组的差异有统计学意义（P < 0.05），2 h暴露组与对照组的差异无统计学意义（P>0.05）。TLR4 mRNA与RelA mRNA两者之间具有相关性（r=0.99，P=0.00）。
结论 矽尘染毒后小鼠肺组织TLR4 mRNA和RelA mRNA的表达上调，提示TLR4/RelA信号通路激活。
Abstract:Objective To investigate the effects of silica dust on the expression of TLR4 mRNA and RelA mRNA in lung tissue of experimental mice.
Methods Forty mice were randomly divided into four groups with 10 mice in each group:control group (not exposed to silica dust), and 2, 4, and 8 h exposure groups (exposed to silica dust for 2, 4, and 8 h every day, respectively). After 20 days of exposure, lung tissues were dissected to detect the expression of TLR4 mRNA and RelA mRNA by RT-qPCR technology.
Results The median expression levels of TLR4 mRNA in the control group and three exposure groups were 0.89, 1.22, 4.89, and 7.21, respectively (χ2=4.93, P=0.02). Compared with the control group, the expression levels of TLR4 mRNA in the 4 h and 8 h exposure groups were both different (P < 0.05), but that in the 2 h exposure group showed no significant changes (P>0.05). The expression levels of RelA mRNA in the control group and three exposure groups were 0.96, 1.33, 2.23, and 3.04, respectively (χ2=6.35, P=0.00). Compared with the control group, the expression levels of RelA mRNA in the 4 h and 8 h exposure groups were both significantly different (P < 0.05), but that in the 2 h exposure group showed no significant changes (P>0.05). The expression levels of TLR4 mRNA and RelA mRNA showed a significant correlation (r=0.99, P=0.00).
Conclusion The elevated expressions of TLR4 mRNA and RelA mRNA in the lung tissue of mice exposed to silica dust indicate the activation of TLR4/RelA signaling pathway.
下载:
