γ射线诱导肠上皮细胞氧化损伤在其凋亡中的作用
Role of Oxidative Damage Induced by Gamma Irradiation in Apoptosis of Intestinal Epithelial Cells
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摘要:
目的 探索γ射线诱导肠上皮细胞氧化损伤在其凋亡中的作用，为进一步阐明其凋亡机制提供科学依据。
方法 选择成年健康大鼠为研究对象，分离并培养大鼠小肠上皮细胞，在室温下以0、1、6、12、18 Gy 60Coγ射线照射24 h，照射完毕继续培养，收集后采用相应的试剂盒检测细胞凋亡情况以及活性氧、超氧化物歧化酶、过氧化氢酶、谷胱甘肽和谷胱甘肽过氧化物酶的水平。
结果 6、12、18 Gy剂量组肠上皮细胞凋亡率分别为7.03%，14.87%和27.95%，均高于对照组（
P < 0.05），且随着照射剂量的增加而升高。与对照组相比：6、12、18 Gy剂量组细胞内活性氧含量分别增加了38.13%，102.53%，215.08%，呈现升高的趋势；谷胱甘肽、谷胱甘肽过氧化物酶含量分别下降了10.26%，55.77%，71.09%和18.32%，32.92%，60.99%，均呈现逐渐降低的趋势；1、6、12、18 Gy剂量组细胞内超氧化物歧化酶含量分别下降了20.18%，42.46%，59.04%，75.71%，呈现降低的趋势；过氧化氢酶含量分别下降了20.75%，48.19%，60.33%，71.15%，呈现逐渐下降的趋势；差异均有统计学意义（P < 0.05）。结论 γ射线照射可以启动组织细胞的脂质过氧化反应，破坏小肠上皮细胞抗氧化系统，导致活性氧堆积，细胞处于氧化应激状态，细胞受损，凋亡率增高。
Abstract:Objective To study the effect of oxidative damage induced by gamma irradiation on the apoptosis of intestinal epithelial cells, and provide a scientific basis for further clarifying relevant mechanism.
Methods Adult healthy rats were used to isolate and culture small intestinal epithelial cells and exposed under 0, 1, 6, 12, and 18 Gy 60Coγ irradiation for 24 h. Apoptosis rate, reactive oxygen species(ROS), superoxide dismutase(SOD), catalase(CAT), glutathione(GSH), and glutathione peroxidase(GSH-px) were detected using corresponding kits.
Results The apoptosis rates of intestinal epithelial cells in the 6, 12, and 18 Gy groups were 7.03%, 14.87%, and 27.95%, respectively, higher than that in the control group(
P < 0.05) and increasing with higher irradiation doses. Compared with the control group, the levels of ROS in intestinal epithelial cells of the 6, 12, and 18 Gy groups increased by 38.13%, 102.53%, and 215.08%, respectively, displaying a rising trend. The levels of GSH(by 10.26%, 55.77%, and 71.09%) and GSH-px(by 18.32%, 32.92%, and 60.99%) decreased with the increase of gamma ray dose. The levels of SOD in intestinal epithelial cells of the 1, 6, 12, and 18 Gy groups decreased by 20.18%, 42.46%, 59.04%, and 75.71%, and the levels of CAT decreased by 20.75%, 48.19%, 60.33%, and 71.15%, respectively, with statistical differences(P < 0.05).Conclusion Gamma ray irradiation can initiate lipid peroxidation of tissues and cells, damage the antioxidant systems of intestinal epithelial cells, and accumulate ROS, which leads to cell oxidative stress, injury, and elevated apoptosis rate.
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