常锐霞, 陈军义, 赵乾龙, 张洁, 魏倩, 党瑜慧, 李芝兰. 丙烯腈亚慢性染毒对雄性大鼠精浆果糖和锌含量的影响[J]. 环境与职业医学, 2016, 33(2): 189-192. DOI: 10.13213/j.cnki.jeom.2016.15417
引用本文: 常锐霞, 陈军义, 赵乾龙, 张洁, 魏倩, 党瑜慧, 李芝兰. 丙烯腈亚慢性染毒对雄性大鼠精浆果糖和锌含量的影响[J]. 环境与职业医学, 2016, 33(2): 189-192. DOI: 10.13213/j.cnki.jeom.2016.15417
CHANG Rui-xia, CHEN Jun-yi, ZHAO Qian-long, ZHANG Jie, WEI Qian, DANG Yu-hui, LI Zhi-lan. Effect of Sub-Chronic Acrylonitrile Exposure on Seminal Fructose and Zinc Contents in Male Rats[J]. Journal of Environmental and Occupational Medicine, 2016, 33(2): 189-192. DOI: 10.13213/j.cnki.jeom.2016.15417
Citation: CHANG Rui-xia, CHEN Jun-yi, ZHAO Qian-long, ZHANG Jie, WEI Qian, DANG Yu-hui, LI Zhi-lan. Effect of Sub-Chronic Acrylonitrile Exposure on Seminal Fructose and Zinc Contents in Male Rats[J]. Journal of Environmental and Occupational Medicine, 2016, 33(2): 189-192. DOI: 10.13213/j.cnki.jeom.2016.15417

丙烯腈亚慢性染毒对雄性大鼠精浆果糖和锌含量的影响

Effect of Sub-Chronic Acrylonitrile Exposure on Seminal Fructose and Zinc Contents in Male Rats

  • 摘要: 目的

    探讨丙烯腈亚慢性染毒对雄性大鼠精浆果糖和锌含量的影响以及果糖、锌含量与精液运动参数的相关关系。

    方法

    SPF级健康成年雄性SD大鼠40只,按体重随机分为对照组(玉米油)、低剂量组(丙烯腈12.5 mg/kg)、中剂量组(丙烯腈25 mg/kg)和高剂量组(丙烯腈50 mg/kg),每组10只。灌胃染毒,1次/d,6 d/周,连续13周。于末次染毒后采用颈椎脱臼法分批处死大鼠,检测大鼠精浆中果糖、锌含量以及附睾精子密度、精子活率、精子活力。

    结果

    中、高剂量染毒组大鼠精囊腺和前列腺系数与对照组比较降低(P=0.045、0.011,P=0.048、0.004)。低、中、高剂量染毒组大鼠精浆果糖、锌含量与对照组比较降低(P=0.002、0.000、0.000;P=0.013、0.016、0.001)。低、中、高剂量染毒组大鼠精子活率与对照组比较降低(P=0.043、0.004、0.014);高剂量染毒组大鼠精子密度、精子活力与对照组比较降低(P=0.007,P=0.031)。精浆果糖、锌含量与精子活率呈正相关(分别r=0.672,P=0.001;r=0.611,P=0.000)。

    结论

    在本实验条件下,丙烯腈亚慢性染毒可以导致雄性大鼠生殖内分泌系统损害,抑制雄性大鼠精囊腺、前列腺分泌果糖和锌,影响精子密度、活率和活力,从而对精子运动及受精能力产生不良影响。

     

    Abstract: Objective

    To explore the effect of sub-chronic acrylonitrile (ACN) exposure on seminal fructose and zinc contents in male rats and the relationship of sperm motion parameters with fructose and zinc contents.

    Methods

    Forty healthy adult male SD rats (SPF degree) were randomly divided by weight into control group (corn oil), low dose group (ACN 12.5 mg/kg), medium dose group (ACN 25 mg/kg), and high dose group (ACN 50 mg/kg), with 10 male rats in each group. Exposure by gavage was conducted once daily, continuous 6 days every week, for 13 weeks. The male rats were sacrificed by cervical dislocation after the last exposure to detect seminal fructose and zinc contents, as well as epididymis sperm density, sperm viability, and sperm motility.

    Results

    Compared to the control group, the seminal vesicle and prostate coefficients were lower in the medium and high dose groups (P=0.045, 0.011; P=0.048, 0.004). The contents of seminal fructose and zinc in each dose group were significantly lower than those in the control group (P=0.002, 0.000, 0.000; P=0.013, 0.016, 0.001). The sperm viability in each exposure group was also lower than that of the control group (P=0.043, 0.004, 0.014); the sperm density and sperm motility in the high dose group were decreased compared to the control group (P=0.007, P=0.031). Sperm viability had positive correlations with seminal fructose and zinc contents (r=0.672, P=0.001; r=0.611, P=0.000).

    Conclusion

    Under current experimental conditions, sub-chronic ACN exposure might cause reproductive endocrine system damage in male rats, inhibit seminal vesicle and prostate secreting fructose and zinc, and influence sperm density, viability, and motility, resulting in adverse effects on sperm motion and fertility.

     

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