翟庆峰, 赵健, 邢杰, 邵丽军, 邱玉刚. 温室作业人员农药暴露致外周血淋巴细胞基因组不稳定表型[J]. 环境与职业医学, 2015, 32(11): 1051-1053,1057. DOI: 10.13213/j.cnki.jeom.2015.15130
引用本文: 翟庆峰, 赵健, 邢杰, 邵丽军, 邱玉刚. 温室作业人员农药暴露致外周血淋巴细胞基因组不稳定表型[J]. 环境与职业医学, 2015, 32(11): 1051-1053,1057. DOI: 10.13213/j.cnki.jeom.2015.15130
ZHAI Qing-feng , ZHAO Jian , XING Jie , SHAO Li-jun , QIU Yu-gang . Phenotypes of Genomic Instability in Peripheral Blood Lymphocytes of Greenhouse Workers with Exposure to Pesticides[J]. Journal of Environmental and Occupational Medicine, 2015, 32(11): 1051-1053,1057. DOI: 10.13213/j.cnki.jeom.2015.15130
Citation: ZHAI Qing-feng , ZHAO Jian , XING Jie , SHAO Li-jun , QIU Yu-gang . Phenotypes of Genomic Instability in Peripheral Blood Lymphocytes of Greenhouse Workers with Exposure to Pesticides[J]. Journal of Environmental and Occupational Medicine, 2015, 32(11): 1051-1053,1057. DOI: 10.13213/j.cnki.jeom.2015.15130

温室作业人员农药暴露致外周血淋巴细胞基因组不稳定表型

Phenotypes of Genomic Instability in Peripheral Blood Lymphocytes of Greenhouse Workers with Exposure to Pesticides

  • 摘要: 目的 分析温室作业农药暴露对菜农外周血淋巴细胞基因组不稳定性的影响及其细胞毒作用。

    方法 对安丘市从事温室蔬菜大棚种植者583 名及普通农民576 名进行问卷调查,根据农药暴露强度,将蔬菜大棚种植组所有调查对象分为低(n=175)、中(n=181)、高(n=212)3 个暴露组。利用胞质分裂阻滞法微核细胞组学试验评价温室农药暴露所致菜农基因组不稳定性和细胞毒性。

    结果 随着农药暴露水平的增加,微核率、核质桥、核芽发生率、细胞坏死率均有增加趋势(均P<0.01),双核细胞率和核分裂指数具有降低趋势(P<0.01),而凋亡细胞未发现明显的变化趋势。与对照组比较,中、高暴露组上述各指标差异皆具有统计学意义(均P<0.05)。

    结论 温室作业人群农药暴露会诱发基因组不稳定表型,并具有较强的细胞毒性。

     

    Abstract: Objective To study the phenotypes of genomic instability in peripheral blood lymphocytes and the cytotoxicity for greenhouse workers with long-term exposure to pesticides.

    Methods Cluster random sampling method was used to select 583 greenhouse workers as pesticide exposure group and 576 common farmers as control group from Anqiu City and to perform a questionnaire survey. According to the level of pesticides exposure, the exposure group was divided into low(n=175), moderate(n=181), and high(n=212) exposure groups. Genomic instability and cytotoxicity of pesticides to peripheral blood lymphocytes were detected by cytokinesis-block micronucleus cytome(CBMN-Cyt) assay.

    Results With higher pesticides exposure level, micronucleus frequency, nucleoplasmic bridges, nuclear buds, and necrotic cell rate were increased(all P<0.01); whereas, binucleated cells and nuclear division index were decreased(both P>0.01); no significant changes in apoptosis cells were observed. Compared with the control group, all the parameters mentioned above of the moderate or high exposure groups were significantly different(all P<0.05).

    Conclusion Exposure of the greenhouse workers to pesticides could cause genomic instability and strong cytotoxicity.

     

/

返回文章
返回