谢长明, 高连连, 赵岩, 陈姣, 汪秀, 蔡德培, 张蕴晖. 体质性发育迟缓男童邻苯二甲酸酯暴露与线粒体DNA拷贝数及氧化损伤的关联性研究[J]. 环境与职业医学, 2015, 32(10): 926-930. DOI: 10.13213/j.cnki.jeom.2015.14774
引用本文: 谢长明, 高连连, 赵岩, 陈姣, 汪秀, 蔡德培, 张蕴晖. 体质性发育迟缓男童邻苯二甲酸酯暴露与线粒体DNA拷贝数及氧化损伤的关联性研究[J]. 环境与职业医学, 2015, 32(10): 926-930. DOI: 10.13213/j.cnki.jeom.2015.14774
XIE Chang-ming , GAO Lian-lian , ZHAO Yan , CHEN Jiao , WANG Xiu , CAI De-pei , ZHANG Yun-hui . Association of Phthalates Exposure with Mitochondrial DNA Copy Number and Oxidative Damage in Boys with Constitutional Delay of Growth and Puberty[J]. Journal of Environmental and Occupational Medicine, 2015, 32(10): 926-930. DOI: 10.13213/j.cnki.jeom.2015.14774
Citation: XIE Chang-ming , GAO Lian-lian , ZHAO Yan , CHEN Jiao , WANG Xiu , CAI De-pei , ZHANG Yun-hui . Association of Phthalates Exposure with Mitochondrial DNA Copy Number and Oxidative Damage in Boys with Constitutional Delay of Growth and Puberty[J]. Journal of Environmental and Occupational Medicine, 2015, 32(10): 926-930. DOI: 10.13213/j.cnki.jeom.2015.14774

体质性发育迟缓男童邻苯二甲酸酯暴露与线粒体DNA拷贝数及氧化损伤的关联性研究

Association of Phthalates Exposure with Mitochondrial DNA Copy Number and Oxidative Damage in Boys with Constitutional Delay of Growth and Puberty

  • 摘要: 目的 采用病例对照研究方法, 观察邻苯二甲酸酯暴露对男童线粒体DNA氧化损伤及线粒体DNA拷贝数的影响, 探索邻苯二甲酸酯影响男性性发育的可能机制。

    方法 将2013-2014 年经上海市某三级甲等医院确诊为体质性发育迟缓的8~15 岁男童纳入研究, 共48 例病例, 匹配以同一年龄段同一医院就诊于外科的正常男童60 例作为对照。采用高效液相色谱-串联质谱法检测病例和对照男童尿液中邻苯二甲酸单丁酯(MnBP)、邻苯二甲酸甲酯(MMP)、邻苯二甲酸乙酯(MEP)邻苯二甲酸(2-乙基己基)单酯(MEHP)、邻苯二甲酸(2-乙基-5 羟基己基)单酯(MEHHP)、邻苯二甲酸(2-乙基-5 氧己基)单酯(MEOHP)浓度。运用t 检验及多元线性回归模型分析邻苯二甲酸酯水平与线粒体DNA氧化损伤、线粒体DNA拷贝数在病例和对照中的差异及两者间的关联。

    结果 病例组男童尿液MnBP、MMP、MEHP、SumDEHP 浓度显著高于正常对照组(P 值分别为0.017、0.008、0.038、0.020)。病例组男童线粒体DNA拷贝数高于对照组(P=0.001), 两组线粒体DNA氧化损伤水平差异无统计学意义。男童尿液MMP、MEHP、SumDEHP 浓度与线粒体DNA拷贝数存在显著正相关(P < 0.05), 但未发现线粒体DNA氧化损伤水平与邻苯二甲酸酯暴露水平间显著关联。

    结论 体质性发育迟缓男童体内邻苯二甲酸酯暴露水平和线粒体DNA拷贝数显著升高, 邻苯二甲酸酯暴露可能是线粒体功能受损的一个危险因素。

     

    Abstract: Objective To investigate the association of phthalates exposure with mitochondrial DNA oxidative damage and copy number in boys based on a case-control study on constitutional delay of growth and puberty (CDGP) and to explore the potential mechanism of phthalates' effect on CDGP.

    Methods A case-control study was conducted in a tertiary class A hospital from 2013 to 2014. The recruited 48 cases were 8-15-year-old boys with diagnosed CDGP, and the 60 controls were boys of same age visiting the surgery department of the hospital. Concentrations of mono-n-butyl phthalate (MnBP), mono-methyl phthalate (MMP), mono-ethyl phthalate (MEP), mono-(2-ethylhexyl) phthalate (MEHP), mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), and mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP) in urine samples were determined using high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS). The relationship between phthalates exposure and mitochondrial DNA copy number and mitochondrial DNA oxidative damage were analyzed by t test and using multiple linear regression models.

    Results Higher mitochondrial DNA copy numbers and higher urinary MnBP, MMP, MEHP, SumDEHP concentrations were detected in the CDGP boys than those in the controls (P=0.001, 0.017, 0.008, 0.038, 0.020, respectively). The mitochondrial DNA oxidative damage level was not different between the cases and the controls. The urinary MMP, MEHP, and sumDEHP concentrations were positively associated with the mitochondrial DNA copy numbers (P < 0.05), but no association was found between the mitochondrial DNA damage level and the phthalates exposure level.

    Conclusion Elevated phthalate levels and mitochondrial DNA copy numbers are found in the CDGP boys compared to the controls. Phthalates exposure might be a risk factor of mitochondrial disfunction.

     

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