燃煤型地方性氟中毒病区8~14岁儿童血清BMP-2、BMP-3水平

吴延莉; 曹发军; 范祥; 徐小东; 向蓉; 曾贝贝; 韦艳; 谢春; 金庭旭; 张华

doi:10.13213/j.cnki.jeom.2015.14709

燃煤型地方性氟中毒病区8~14岁儿童血清BMP-2、BMP-3水平

Serum BMP-2 and BMP-3 Levels in Children of 8-14 Years Old in Coal-Burning Endemic Fluorosis Areas

摘要

摘要: 目的观察燃煤型氟中毒病区儿童血清骨形态发生蛋白-2(BMP-2)和骨形态发生蛋白-3(BMP-3)的表达情况。

方法对贵州省六盘水市水城县陡箐乡某两所小学(氟病区)366名和花嘎乡某所小学(对照区)120名8~14岁在校儿童进行氟斑牙和尿氟浓度调查;根据知情同意原则,选取氟病区(根据氟斑牙的分级)115例和对照区57例在校儿童,收集血清,检测血清BMP-2、BMP-3的浓度。

结果氟病区儿童氟斑牙检出率为61.5%,对照区儿童无氟斑牙发生;氟病区儿童尿氟浓度M(P₂₅,P₇₅) 0.77(0.536,1.136)mg/L较对照区0.49(0.370,0.660)mg/L高,差异均有统计学意义(χ²_氟斑牙=265.130,P_氟斑牙< 0.05;Z_尿氟=-7.716,P_尿氟< 0.05)。氟病区儿童血清BMP-2、BMP-3浓度M(P₂₅,P₇₅)为16.40(13.51,27.08)、155.39(132.57,177.95)ng/L较对照区儿童血清BMP-2、BMP-3浓度18.73(14.89,25.84)、163.67(149.18,190.74)ng/L低,差异均有统计学意义(Z_BMP-2=-2.023,P_BMP-2<0.05;Z_BMP-3=-2.736,P_BMP-3<0.01)。氟病区13~14岁组儿童血清BMP-3浓度139.52(122.53,159.07)ng/L较对照组174.05(154.15,195.03)ng/L低,差异有统计学意义(Z=-2.967,P < 0.01)。氟病区儿童血清BMP-2、BMP-3浓度在不同程度氟斑牙之间差异无统计学意义(χ²_BMP-2=4.680,P_BMP-2=0.322;χ²_BMP-3=2.649,P^BMP-3=0.618)。氟病区儿童血清BMP-2、BMP-3浓度在不同尿氟浓度水平之间差异无统计学意义(χ²BMP-2=1.292,P_BMP-2=0.731;χ²BMP-3=1.440,P_BMP-3=0.696)。

结论贵州省六盘水市氟病区儿童血清BMP-2、BMP-3浓度较低,氟可能影响血清BMP-2、BMP-3表达。

Abstract: Objective To observe the expression of bone morphogenetic protein-2 (BMP-2) and bone morphogenetic protein-3 (BMP-3) in the serum of children in coal-burning endemic fluorosis areas.

Methods The study enrolled 366 children from two primary schools of Tujing Village (endemic fluorosis area) and 120 children from one primary school of Huaga Village (control area), Shuicheng County, Liupanshui City, Guizhou Province. All children were 8-14 years old and received a survey on dental fluorosis and urinary fluoride. Based upon informed consent, 115 children in the endemic fluorosis area categorized by dental fluorosis grade and 57 children in the control area were selected for serum sample collection to determine concentrations of serum BMP-2 and BMP-3.

Results The incidence rate of dental fluorosis in the endemic fluorosis area was 61.5% and dental fluorosis was not found in the control children (χ²=265.130, P < 0.05). The children's average urinary fluoride concentration in the fluorosis areamedian (P₂₅, P₇₅) 0.77(0.536, 1.136)mg/L was higher (Z=-7.716, P < 0.05) than that in the control area0.49(0.370, 0.660)mg/L (Z=-7.716, P < 0.05). The average concentrationsmedian (P₂₅, P₇₅) of serum BMP-2 and BMP-3 of children in the endemic fluorosis area16.40(13.51, 27.08)ng/L and 155.39(132.57, 177.95)ng/L, respectively were lower than those in the control area18.73(14.89, 25.84)ng/L and 163.67(149.18, 190.74)ng/L, respectively (Z_BMP-2=-2.023, P_BMP-2< 0.05; Z_BMP-3=-2.736, P_BMP-3< 0.01, respectively). The average concentration of serum BMP-3 in the children of 13-14 age group in the endemic fluorosis area139.52(122.53, 159.07)ng/L was lower than that of the children at same age group in the control area174.05(154.15, 195.03)ng/L (Z=-2.967, P < 0.01). No differences were found in serum levels of BMP-3 and BMP-2 among the children in the endemic fluorosis area categorized by dental fluorosis degree (χ²BMP-2=4.680, P_BMP-2=0.322; χ²_BMP-3=2.649, P_BMP-3=0.618, respectively) and urinary fluoride (χ²_BMP-2=1.292, P_BMP-2=0.731; χ²_BMP-3=1.440, P_BMP-3=0.696, respectively).

Conclusion Low concentrations of serum BMP-2 and BMP-3 are measured in the children in fluorine disease areas in Liupanshui City of Guizhou Province, indicating that fluoride may impact the expression of BMP-2 and BMP-3 in serum.

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