被动吸烟对孕妇尿中4种多环芳烃代谢物浓度的影响
Effects of Passive Smoking on Levels of Four Urinary Polycyclic Aromatic Hydrocarbon Metabolites in Pregnant Women
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摘要:
目的 以尿中2-羟基萘(2-NAP)、2-羟基芴(2-FLU)、9-羟基菲(9-PHE)和1-羟基芘(1-PYR)作为生物标志物,评价太原市孕妇体内多环芳烃(PAHs)暴露水平,探讨被动吸烟对其浓度的影响。 方法 2009年4月-2010年4月,抽取太原市某医院住院分娩孕妇287人,对其工作生活环境等情况进行问卷调查。将符合纳入标准的264名孕妇根据被动吸烟指数等级分为3组:0、1~49、≥ 50组,收集各组产前尿样,利用高效液相色谱-荧光检测法测定尿中4种PAHs的羟基代谢产物(OH-PAHs)水平。 结果 符合纳入标准的孕妇尿样中均可检测到2-羟基萘、2-羟基芴、9-羟基菲和1-羟基芘,浓度中位数分别为0.47、0.24、0.23和0.10μg/mmol Cr。除9-羟基菲外,3组尿中2-羟基萘、2-羟基芴、1-羟基芘的差异均有统计学意义(P < 0.05);多元线性回归分析显示,被动吸烟、使用煤炉、采暖期、汽修厂加油站是影响孕妇尿中4种OH-PAHs水平的主要因素。 结论 被动吸烟可以增加孕妇尿中4种OH-PAHs含量,其中2-羟基萘可以作为反映被动吸烟对孕妇体内多环芳烃暴露水平影响的最佳指标。 Abstract:Objective To evaluate the exposure levels of prenatal polycyclic aromatic hydrocarbons (PAHs) in Taiyuan, with urinary 2-hydroxynathalene (2-NAP), 2-hydroxyfluorene (2-FLU), 9-hydroxyphenanthrene (9-PHE), and 1-hydroxypyrene (1-PYR) as biomarkers, thus explore if the exposure levels are influenced by passive smoking. Methods Pregnant women (n=287) admitted to a hospital for delivery were recruited and inquired about the living and working conditions during the period of April 2009 to April 2010 in Taiyuan. A total of 264 pregnant women joined the study and were divided into three groups according to the passive smoking index (0, 1-49, ≥ 50 groups). The urine samples of the selected pregnant women were collected before delivery and four urinary PAHs metabolites (OH-PAHs) were measured by high performance lipid chromatography-fluorescence detection. Results 2-NAP, 2-FLU, 9-PHE, and 1-PYR were detected in the urine of involved pregnant women, and the median concentrations were 0.47, 0.24, 0.23, and 0.10 μg/mmol Cr respectively. The differences were statistically significant in urinary concentrations of 2-NAP, 2-FLU, and 1-PYR among the three groups (P < 0.05). However, 9-PHE showed no significant difference. Several factors were found to have critical influences on four urinary OH-PAHs, such as passive smoking, using coal stove, heating period, living near garages and gas stations by multiple linear regression. Conclusion Passive smoking might contribute to elevated levels of four urinary OH-PAHs, of which 2-NAP is the best marker for evaluating maternal prenatal PAHs exposure levels associated with passive smoking.
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