超重环境下微波辐射对大鼠的损伤效应

姜艳霞; 兰兰; 曹颜东; 罗军; 徐俊杰; 张巍; 朱文赫

doi:10.13213/j.cnki.jeom.2015.14249

摘要: 目的探讨超重环境下微波辐射大鼠的损伤情况。

方法大鼠随机分为正常对照组、微波组和超重微波组,每组各12只。超重微波组先在6G超重环境下持续5 min后,接着在6G超重环境下实施200 mW/cm²的微波辐射5 min。

结果超重微波组与正常对照组、微波组血清一氧化氮(NO)差异均有统计学意义(P<0.05);超重微波组、微波组的超氧阴离子、一氧化氮合酶(NO synthetase,NOS)与正常对照组比较,差异均有统计学意义(q₁=13.587 6,q₂=25.923 5;P<0.05);微波组与超重微波组超氧阴离子比较,差异有统计学意义(q=11.487 6,P<0.05);微波组过氧化氢酶(catalase,CAT)与正常对照组、超重微波组比较,差异有统计学意义(q₁=7.840 4,q₂=5.339 1;P<0.05);微波组谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-pox)与正常对照组比较,差异有统计学意义(q₁=5.900 1,P<0.05);超重微波组、微波组GSH与正常对照组比较,差异有统计学意义(q₁=8.948 7,q₂=7.114 5;P<0.05)。在肝组织匀浆中,超重微波组、微波组CAT与正常对照组比较,差异有统计学意义(q₁=3.381 5,q₂=2.970 9;P<0.05);超重微波组、微波组GSH-pox与正常对照组比较,差异有统计学意义(q₁=3.382 2,q₂=5.196 8;P<0.05);超重微波组NOS与微波组、正常对照组比较,差异有统计学意义(q₁=4.239 3,q₂=4.088 3;P<0.05);微波组GSH与正常对照组、超重微波组比较,差异有统计学意义(q₁=11.6634,q₂=15.983 1;P<0.05)。显微镜下观察超重微波组的肝细胞轻度肿胀,间隙加大,肝血窦变宽。

结论超重环境下微波辐射导致的损伤可能是超氧阴离子增高所致。

Abstract: Objective To examine the injury to rats induced by microwave radiation in hypergravity setting.

Methods Rats were randomly divided into control, microwave, and hypergravity + microwave groups. There were 12 rats in each group. The hypergravity+microwave group was firstly exposed to a 6G setting for 5 min, and then exposed to 200 mW/cm² microwave under the 6G environment for another 5 min.

Results Serum nitric oxide (NO) in the control and microwave groups were significantly different from that of the hypergravity+microwave group (P<0.05). The levels of superoxide anion and nitric oxide synthase (NOS) in the microwave and hypergravity+microwave groups were significantly different from those of the control group (q₁=13.587 6, q₂=25.923 5, P<0.05); similar differences were also found between the microwave group and the hypergravity+microwave group (q=11.487 6, P<0.05). The level of catalase (CAT) in the microwave group was different as compared to those of the control and the hypergravity+ microwave groups (q₁=7.840 4, q₂=5.339 1, P<0.05). The level of glutathione peroxidase (GSH-pox) in the microwave group was obviously different as compared to that of the control group (q=5.900 1, P<0.05). The level of glutathione (GSH) in the hypergravity+ microwave and the microwave groups were significantly different from that of the control group (q₁=8.9487, q₂=7.114 5; P<0.05). In the liver homogenate, the levels of CAT and GSH-pox in the microwave and the hypergravity+microwave groups were significantly different as compared to those of the control group (q₁=3.381 5, q₂=2.970 9; P<0.05); the level of NOS in the hypergravity microwave group was obviously different as compared to that of the microwave and the control groups (q₁=4.239 3, q₂=4.088 3; P<0.05); the level of GSH in the microwave group was obviously different from those of the hypergravity+microwave and control groups (q₁=11.663 4, q₂=15.983 1, P<0.05). Liver cells were slightly swollen, intercellular space grew bigger, and liver sinusoid became wider under the microscope observation.

Conclusion The mechanism of microwave radiation injury in hypergravity setting is possibly due to increased superoxide anion.

超重环境下微波辐射对大鼠的损伤效应

Microwave Radiation Injury to Rats in Hypergravity Setting