卜德云, 牛照地, 田大年, 朱玲勤, 刘贺荣, 纪文武, 周健, 杨惠芳. 纳米SiO2与介孔SiO2对脐静脉血管内皮细胞毒性作用的比较研究[J]. 环境与职业医学, 2014, 31(12): 936-940. DOI: 10.13213/j.cnki.jeom.2014.0228
引用本文: 卜德云, 牛照地, 田大年, 朱玲勤, 刘贺荣, 纪文武, 周健, 杨惠芳. 纳米SiO2与介孔SiO2对脐静脉血管内皮细胞毒性作用的比较研究[J]. 环境与职业医学, 2014, 31(12): 936-940. DOI: 10.13213/j.cnki.jeom.2014.0228
BU De-yun , NIU Zhao-di , TIAN Da-nian , ZHU Ling-qin , LIU He-rong , JI Wen-wu , ZHOU Jian , YANG Hui-fang . Comparative Study on Silica Nanoparticles and Mesoporous Silica Induced Toxicity to Human Umbilical Vein Endothelial Cells[J]. Journal of Environmental and Occupational Medicine, 2014, 31(12): 936-940. DOI: 10.13213/j.cnki.jeom.2014.0228
Citation: BU De-yun , NIU Zhao-di , TIAN Da-nian , ZHU Ling-qin , LIU He-rong , JI Wen-wu , ZHOU Jian , YANG Hui-fang . Comparative Study on Silica Nanoparticles and Mesoporous Silica Induced Toxicity to Human Umbilical Vein Endothelial Cells[J]. Journal of Environmental and Occupational Medicine, 2014, 31(12): 936-940. DOI: 10.13213/j.cnki.jeom.2014.0228

纳米SiO2与介孔SiO2对脐静脉血管内皮细胞毒性作用的比较研究

Comparative Study on Silica Nanoparticles and Mesoporous Silica Induced Toxicity to Human Umbilical Vein Endothelial Cells

  • 摘要: 目的 探讨并比较纳米SiO2 (nano-SiO2)与介孔SiO2 (SBA-15)对人脐静脉血管内皮细胞(HUVEC)的毒性效应及氧化损伤作用。

    方法 将nano-SiO2、SBA-15 按照不同作用浓度(0.00、6.25、12.50、25.00、50.00、100.00、200.00 μg/mL)对HUVEC 进行染毒,作用24 h 后,测定HUVEC 的增殖抑制情况、培养液中乳酸脱氢酶(LDH)的活性,及细胞内活性氧(ROS)的表达水平。

    结果 与阴性对照组(0 μg/mL)比较,nano-SiO2、SBA-15 组细胞抑制率均升高(P<0.05),且具有明显的剂量-反应关系;低浓度(12.50 μg/mL)时SBA-15 组对细胞抑制率高于nano-SiO2 组;随着染毒浓度增加,nano-SiO2 组对细胞抑制率高于SBA-15 组(P<0.05)。与阴性对照组比较,nano-SiO2、SBA-15 组LDH、ROS 水平均升高(P<0.05),且随着染毒浓度的升高呈现明显的剂量-反应关系(P<0.05);各剂量SBA-15 染毒组的LDH和ROS 水平均小于同剂量nano-SiO2 染毒组(P<0.05)。

    结论 nano-SiO2 与SBA-15 对血管内皮细胞均存在细胞毒性效应,氧化损伤可能是其细胞毒性机制之一。与nano-SiO2 组相比,SBA-15 细胞毒性较低。

     

    Abstract: Objective To study and compare silica nanoparticles (nano-SiO2) and mesoporous silica (SBA-15) induced cytotoxicity and oxidative damage on human umbilical vein endothelial cells (HUVEC).

    Methods HUVEC were exposed to nano-SiO2 and SBA-15 with different concentrations (0.00, 6.25, 12.50, 25.00, 50.00, 100.00, 200.00 μg/mL) for 24 hours. The inhibition rates of cell proliferation, expression levels of cell reactive oxygen species (ROS) and lactate dehydrogenase (LDH) activity in the medium supernatant were measured.

    Results Compared with the negative control group (0 μg/mL), the inhibition rate of the nano-SiO2 group and the SBA-15 group were significantly increased (P<0.05) and in an distinct dose-effect manner. At a low concentration of 12.50 μg/mL the inhibition rates of the SBA-15 group were higher than those of the nano-SiO2 group, the pattern reversed in high concentration range. Compared with the negative control group (0 μg/mL), the LDH activity and intracellular ROS levels of the nano-SiO2 group or the SBA-15 group were significantly increased (P<0.05), also showing a distinct dose-effect relationship. The LDH activity and the ROS levels of the SBA-15 group at various doses were lower than those of the nano-SiO2 group at the same dose.

    Conclusion Both nano-SiO2 and SBA-15 impose toxic effects on HUVEC. Oxidative damage may be one mechanism of cytotoxicity induced by silica materials. SBA-15 shows lower cytotoxicity comparing with silica nanoparticles.

     

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