1,3-丁二烯作业工人的DNA修复能力
DNA Repair Capacity of Workers Exposed to 1,3-Butadiene
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摘要:
目的 探讨DNA修复能力与1,3-丁二烯(BD)职业暴露致外周血淋巴细胞遗传学损伤的关联性。 方法 收集个人职业史、年龄、性别、吸烟和饮酒状况等信息,气相色谱法检测作业环境的BD浓度,利用染色体断裂试验评价60 名职业BD暴露工人和60 名非暴露工人的外周血淋巴细胞对诱变剂博莱霉素所致DNA损伤的修复能力。 结果 作业区空气中BD浓度为1.8(0.59~2.76)mg/m3。职业BD暴露组染色体断裂率(1.06& #177;0.41)%高于对照组(0.85& #177;0.36)%,P<0.01。职业BD暴露人群中饮酒者的b/c 值高于不饮酒者(P<0.05)。 结论 DNA修复能力的下降可能是BD致癌过程中的重要生物学事件。 Abstract:Objective To explore the relationship between DNA repair capacity (DRC) and chromosomal damage induced by 1, 3-butadiene (BD). Methods Personal information including occupational history, age, gender, smoking, and drinking status was collected using questionnaires. Gas chromatography was used to detect the BD level in the selected working environment. DRC in peripheral blood lymphocytes among 60 BD-exposed workers and 60 non-exposed controls was measured by the repair rate of bleomycin-induced DNA damage using chromatid breakage assay. Results The average concentration of BD in the working environment was 1.80 (0.59-2.76) mg/m3. The chromatid break rates (break event per cell, b/c) were higher in the BD-exposed workers than those in the controls (1.06& #177;0.41)% vs. (0.85& #177;0.36)%, P<0.01. The b/c of alcohol drinkers in the BD-exposed workers was higher than that of non-drinkers. Conclusion Decreased DRC may be an important biological event in BD carcinogenesis.
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