郑姗姗, 王素华, 王春虾, 余艳琴, 张营, 武婧, 姜华, 刘建国, 王玉, 李秋玲. 稀土氧化钕粉尘对大鼠肺灌洗液中细胞总数变化和蛋白含量的影响[J]. 环境与职业医学, 2014, 31(10): 788-792. DOI: 10.13213/j.cnki.jeom.2014.0192
引用本文: 郑姗姗, 王素华, 王春虾, 余艳琴, 张营, 武婧, 姜华, 刘建国, 王玉, 李秋玲. 稀土氧化钕粉尘对大鼠肺灌洗液中细胞总数变化和蛋白含量的影响[J]. 环境与职业医学, 2014, 31(10): 788-792. DOI: 10.13213/j.cnki.jeom.2014.0192
ZHENG Shan-shan , WANG Su-hua , WANG Chun-xia , YU Yan-qin , ZHANG Ying , WU Jing , JIANG Hua , LIU Jian-guo , WANG Yu , LI Qiu-ling . Effects of Rare Earth Neodymium Oxide Dust on Total Cell Number and Protein Content in Brocho Alveolar Lavage Fluid of Rats[J]. Journal of Environmental and Occupational Medicine, 2014, 31(10): 788-792. DOI: 10.13213/j.cnki.jeom.2014.0192
Citation: ZHENG Shan-shan , WANG Su-hua , WANG Chun-xia , YU Yan-qin , ZHANG Ying , WU Jing , JIANG Hua , LIU Jian-guo , WANG Yu , LI Qiu-ling . Effects of Rare Earth Neodymium Oxide Dust on Total Cell Number and Protein Content in Brocho Alveolar Lavage Fluid of Rats[J]. Journal of Environmental and Occupational Medicine, 2014, 31(10): 788-792. DOI: 10.13213/j.cnki.jeom.2014.0192

稀土氧化钕粉尘对大鼠肺灌洗液中细胞总数变化和蛋白含量的影响

Effects of Rare Earth Neodymium Oxide Dust on Total Cell Number and Protein Content in Brocho Alveolar Lavage Fluid of Rats

  • 摘要: 目的 研究稀土氧化钕粉尘对大鼠肺灌洗液(BALF)中细胞总数和蛋白含量的动态变化。

    方法 选用SPF 级健康成年雄性SD大鼠195 只,体重(200& #177;16)g,采用气管非暴露插管灌注法染尘。不同染毒时间组分别采用100 mg/kg 稀土氧化钕粉尘悬液0.8 mL 和0.9%生理盐水0.8 mL 一次性染尘,分别于第3、7、14、21、28 天各处死15 只实验组大鼠和10 只对照组大鼠;不同剂量组按25、50、100、150 mg/kg 剂量0.8 mL 一次性染尘,在染毒第28 天处死各实验组大鼠15 只,对照组处死10 只(由于实验组染毒时间均为28 d,所以设同一对照组为不同染毒剂量组中实验组对照)。取大鼠支气管肺泡灌洗液,用白细胞计数板在显微镜下对细胞总数进行计数。ELISA法分别测定不同染毒时间组和不同染毒剂量组大鼠BALF 中上清液和巨噬细胞蛋白的含量。

    结果 不同染毒时间组和不同染毒剂量组中实验组大鼠较对照组大鼠BALF 中细胞总数均升高(P< 0.05);且在各染毒时间组中细胞总数出现先增加后减少,在第14 天达到最低值后又呈增加的变化趋势,但均高于对照组(P< 0.05)。在不同染毒剂量组中细胞总数随着染毒剂量增加而逐渐增多,细胞总数均高于对照组(P< 0.05)。不同染毒时间组中实验组大鼠较对照组大鼠BALF 上清和巨噬细胞中的蛋白含量均明显增高(P< 0.01)。不同染毒剂量组中实验组大鼠较对照组大鼠BALF 上清液和巨噬细胞中的蛋白含量均明显增高(P <0.01)。不同染毒时间组大鼠BALF 上清液和巨噬细胞蛋白含量出现先增加后减少,在第14 天达到最低值后又呈增加的变化趋势,但均高于对照组(P< 0.05)。不同染毒剂量组中大鼠BALF 上清液和巨噬细胞蛋白含量随着剂量增加而逐渐增多,蛋白含量均高于对照组(P< 0.05)。

    结论 稀土氧化钕粉尘进入大鼠肺组织后,BALF 中细胞总数以及上清液和巨噬细胞中蛋白含量均发生了明显的变化。

     

    Abstract: Objective To examine the dynamic changes in total cell number and protein content in broncho alveolar lavage fluid(BALF) of rats with exposure to rare earth neodymium oxide dust(RENOD).

    Methods A total of 195 healthy adult male SD rats SPF grade; body weight,(200& #177;16) g were treated by unexposed tracheal intubation perfusion. The different exposure time groups were exposed to 0.8 mL of 100 mg/kg RENOD suspension(experimental rats) and 0.8 mL of 0.9% normal saline(control rats); 15 experimental and 10 control rats were neutralized at each designed interval(at the 3rd, 7th, 14th, 21st, and 28th days after dust exposure). The different exposure dose groups were treated with 0.8 mL of RENOD at doses of 25, 50, 100, and 150 mg/kg, respectively; 15 experimental and 10 control rats were neutralized after 28 d(because the full exposure time was 28 d for the time and dose groups, the single control group was applicable to both model groups). The total cell numbers in BALF of rats were counted using a nageotte counting chamber under microscope. The contents of protein were measured by ELISA in supernatant and macrophage in BALF of rats in both the time and the dose exposure groups.

    Results The total cell numbers in the experimental rats of both the different exposure time groups and the different exposure dose groups were significantly increased than those of the control rats(P< 0.05). Specifically, the total cell numbers of experimental rats in the different exposure time groups were first increased, then declined to the lowest at 14 d, and increased again thereafter, but the values at each time point were higher than those of the control rats(P< 0.05). In the different exposure dose groups, the total cell numbers of the experimental rats were gradually increased with the increasing dose and higher than those in the control group(P< 0.05). In both the different exposure time groups and the different exposure dose groups, the contents of protein in BALF supernatant and macrophage of experimental rats were significantly increased than those of the control rats(P< 0.01). The contents of protein in BALF supernatant and macrophage of the experimental rats in the different exposure time groups were first increased, then declined to the lowest at 14 d, and increased again thereafter, but were all higher than those of the control rats(P< 0.05). In the different exposure dose groups, the contents of protein of the experimental rats were gradually increased with the increasing dose and were higher than those of the control group(P< 0.05).

    Conclusion Rat lung tissues exposed to rare earth neodymium oxide dust present significant changes in total cell number and protein content in macrophage and supernatant of BALF.

     

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