纳米碳酸钙亚慢性染毒对大鼠神经行为的影响
Neurobehavior of Rats Following Subchronic Exposure to Calcium Carbonate Nanoparticles
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摘要:
目的 通过观察纳米碳酸钙亚慢性染毒对大鼠自发活动和学习记忆能力的影响,初步探讨纳米碳酸钙的神经毒性。 方法 选取50只健康雄性SD大鼠,随机分为溶剂对照组、微米碳酸钙组(200 mg/kg)和纳米碳酸钙12.5、50、200 mg/kg组5组,每组10只。滴鼻法每天染毒1次,每周5 d,间歇2 d,持续12周;采用旷场试验和Morris水迷宫试验测试各组大鼠自发活动和学习记忆功能。 结果 微米碳酸钙和纳米碳酸钙组大鼠精神萎靡,但各组间大鼠体质量和脑体比差异均无统计学意义(P>0.05)。旷场试验,各组大鼠在暗箱中中央活动时间、中央运动总路程、站立次数差异均无统计学意义(F值分别为0.799、0.623和0.251, P值均大于0.05)。Morris水迷宫试验,各组大鼠平均潜伏期、平均总路程、穿越平台次数、平台象限滞留时间差异均无统计学意义(F值分别为0. 475、0.345、0.046和0.252, P值均大于0.05)。 结论 在本实验剂量条件下,纳米碳酸钙对大鼠自发活动和学习记忆能力无明显影响。 Abstract:Objective To assess the neurotoxicity of calcium carbonate nanoparticles (nano-CaCO3) by observing the changes in spontaneous activity and learning and memory of rats with subchronic exposure to nano-CaCO3. Methods Fifty healthy male SD rats were randomly divided into a solvent control group (saline), a micro-CaCO3 group (200 mg/kg), and three nanoCaCO3 groups (12.5, 50, and 200 mg/kg), 10 rats in each group. The test substances were administered to rats by nasal instillation five times per week for twelve weeks. Open field test and Morris water maze were performed to test the spontaneous activity and the le arning and memory ability of rats. Results Depression was found in the rats exposed to micro-CaCO3 or nano-CaCO3. There was no significant difference in body weight and brain-to-body weight ratio among all groups (P>0.05). The results of open field test showed no significant differences in time spent in center, total distance traveled, and times of vertical activity among all groups (F values were 0.799, 0.623, and 0.251, all P>0.05). The results of Morris water maze showed no significant differences in average escape latency, average path length, times of passing through platform, and residence time in platform quadrant among all groups (F values were 0.475, 0.345, 0.046, and 0.252, all P>0.05). Conclusion No obvious damage to spontaneous activity and learning and memory of rats exposed to nano-CaCO3 is found in this experiment setting.
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