垃圾焚烧区周边居民血浆中细胞因子的表达水平及其影响因素
Plasma Levels of Cytokines and the Risk Factors in Residents Living in the Vicinity of an Watse Incinerator
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摘要:
目的 探讨垃圾焚烧区周边居民外周血中细胞色素P4501A1(CYP1A1)基因mRNA表达水平和血浆中细胞因子白介素(IL) -6、IL-17A、IL-17F、IL-21、IL-23的表达水平。 方法 选择居住社区位于垃圾焚烧厂烟囱下风向<10 km范围内某社区的男性居民42名为暴露组;居住区距垃圾焚烧区>10 km且无明显环境接触垃圾焚烧污染物的44名男性居民为对照组。采用半定量逆转录-聚合酶链反应检测研究对象外周血中CYP1A1基因mRNA相对表达水平;Bio-PlexTM悬液芯片测定研究对象血浆中IL-6、IL-17A、IL-17F、IL-21、IL-23细胞因子的浓度。 结果 暴露组人群外周血CYP1A1基因mRNA相对表达水平高于对照组人群的平均水平(P<0.05)。暴露组人群血浆中细胞因子IL-17F、IL-21、IL-23的浓度均高于对照组(P<0.05)。细胞因子IL-17F、IL-21、IL-23的浓度经自然对数转换后分别与暴露、年龄、体质指数等因素做多元线性回归分析,发现暴露与IL-17F、IL-23表达水平的升高有关(P<0.05)。 结论 暴露组人群外周血CYP1A1基因mRNA表达水平高于对照组人群的平均水平,且两组人群血浆中IL-17F、IL-23的表达水平亦有差异。 Abstract:Objective To measure the levels of a panel of cytokines interleukin-6(IL-6), IL-17A, IL-17F, IL-21, IL-23 in plasma and the expression of cytochrome P450 1A1(CYP1A1) gene in peripheral leukocytes of residents living around an waste in cinerator. Methods This present study recruited 42 male residents from a community within 10 km downwind from the waste incinerator (exposure group) and 44 male residents from a community beyond 10 km from the waste incinerator and without known industrial pollution sources (control group). CYP1A1 gene expressions in participants' leukocytes were determined with semi-quantitative reverse transcriptase polymerase chain reaction, and plasma cytokine (IL-6, IL-17A, IL-17F, IL-21, IL-23) levels were measured by Bio-PlexTM Cytokine Panel. Results The relative CYP1A1 gene expression levels in the exposure group were significantly higher than those in the control group (P<0.05). Also, the plasma levels of IL-17F, IL-21, and IL-23 in the exposure group were significantly higher (P<0.05). After adjusting for age, body mass index, smoking, and alcohol consumption, the transformed levels of IL-17F and IL-23 were associated with exposure (P<0.05) in the multi linear regression analyses. Conclusion The mRNA expressions of CYP1A1 and the plasma levels of IL-17F and IL-23 in residents living in the vicinity of the waste in cinerator are remarkably higher than those of the residents living further away from the waste incinerator.
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